Pharmacological investigation of spreading depression propagation in rat neocortical tissues
von Aziza El Harrak
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[1.] Aeh/Fragment 013 01 - Diskussion Zuletzt bearbeitet: 2014-04-29 22:23:28 Schumann | Aeh, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Wernsmann et al 2006 |
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Untersuchte Arbeit: Seite: 13, Zeilen: 1-7 |
Quelle: Wernsmann et al 2006 Seite(n): 1104, Zeilen: l.col: 22-31 |
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A glass electrode filled with 2 m KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the temporal neocortical slices (layer III). A high-pressure pulse was applied to inject in the tissue an amount of K+ sufficient to induce CSD (tip diameter, 2 μm; injection pressure, 0.5–1.0 bar applied for 200–300 ms, two separate injections, 1–3 nL per pulse, 2–5 mm apart from nearby recording electrodes). CSD were evaluated with respect to their amplitudes, duration and velocity rates. Duration of DC potential fluctuation width was measured at its half-maximal amplitude. | A glass electrode filled with 2 m KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the temporal neocortical slices (layer I–II) and hippocampal tissues (CA1 area). A high-pressure pulse was applied to inject in the tissue an amount of K+ sufficient to induce SD (tip diameter, 2 μm; injection pressure, 0.5–1.0 bar applied for 200–300 ms, up to two separate injections, 1–3 nL per pulse, 2–5 mm apart from nearby recording electrodes). SD were evaluated with respect to their amplitudes, duration and velocity rates. Duration of DC potential fluctuation width was measured at its half-maximal amplitude. |
The source is not mentioned. |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Schumann, Zeitstempel: 20140429222416
Letzte Bearbeitung dieser Seite: durch Benutzer:Schumann, Zeitstempel: 20140429222416