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Quelle: Katzaki 2009
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The need to screen the whole genome at a resolution that surpassed the existing technologies led to the implementation of microarray based CGH. The principle is very similar to that employed for traditional CGH, where two differentially labelled specimens are co-hybridized in the presence of Cot1 DNA. However, instead of metaphase spreads, the hybridization targets are DNA substrates immobilized on a glass slide. [5] [6] [7] Subsequently, the arrays are scanned and the resultant data are analyzed by software that computes the log 2 ratios for a variety of copy number differences between a patient and reference sample (Fig. 2).

Fig. 2⏐ Schematic representation of an array-CGH experiment. a) Test and reference DNA are differentially labelled, co-precipitated and hybridised to an array. b) and c) After wash procedures, the slides are analysed through a scanner and fluorescence intensities of each probe are determined. d) After imaging processing and data normalization, the log2 ratios of the probes are plotted as a function of chromosomal position. Probes with a value of zero represent equal fluorescence intensity ratio between sample and reference. Each dot represents a single probe spotted on the array. In this representation, copy number loss shift the ratio to the left and copy number gains shift the ratio to the right.

Fig. 4⏐ Schematic representation of an array-CGH experiment. a) Test and reference DNA are differentially labelled, co-precipitated and hybridised to an array. b) and c) After wash procedures, the slides are analysed through a scanner and fluorescence intensities of each probe are determined. d) After imaging processing and data normalization, the log2 ratios of the probes are plotted as a function of chromosomal position. Probes with a value of zero represent equal fluorescence intensity ratio between sample and reference. Each dot represents a single probe spotted on the array. In this representation, copy number loss shift the ratio to the left and copy number gains shift the ratio to the right. (d) The need to screen the whole genome at a resolution that surpassed the existing technologies led to the implementation of microarray based CGH. The principle is very similar to that employed for traditional CGH, where two differentially labeled specimens are cohybridized in the presence of Cot1 DNA; however, instead of metaphase spreads, the hybridization targets are DNA substrates immobilized on a glass slide.5-7 Subsequently, the arrays are scanned and the resultant data are analyzed by software that computes the log 2 ratios for a variety of copy number differences between a patient and reference sample (Fig. 4)
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