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[1.] Analyse:Kf/Fragment 131 07 - Diskussion
Bearbeitet: 17. October 2012, 21:24 Anonym69
Erstellt: 20. April 2012, 17:10 (Anonym69)
Fragment, Kf, SMWFragment, Schutzlevel, Verschleierung, Yildirim 2002, ZuSichten

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Verschleierung
Bearbeiter
anonym69
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Untersuchte Arbeit:
Seite: 129, Zeilen: 7
Quelle: Yildirim_2002
Seite(n): 131, Zeilen: -
In the present investigation 79 streptococcal cultures isolated from subclinical mastitis of 54 cattle from seven dairy farms in Hesse, Germany, were

comparatively investigated by using conventional and molecular studies. The cultures were identified as Streptococcus agalactiae, belonging to Lancefield’s serological group B by determination of biochemical and serological properties and by polymerase chain reaction (PCR) amplification of species specific parts of the 16S ribosomal DNA, the 16S-23S rDNA intergenic spacer region and the CAMP factor gene cfb. The investigated group B streptococci were further characterized serologically for specific polysaccharide and protein antigens, partly supported by PCR-amplification of the respective genes. Serotyping the isolates revealed a predominance of the surface protein X , either alone or in combination with polysaccharide antigen Ia. Six group B streptococci from one farm displayed the serotype pattern III/Rib. Two isolates from another farm showed the serotype pattern Ib/cαβ. The remaining cultures were non typable. Additional phenotypic properties which allowed a differentiation of the various cultures were the grade of pigmentation, the growth properties in fluid media and softagar, the surface hydrophobicity, the ability to hemagglutinate rabbit erythrocytes and the resistance reaction to tetracycline and minocyclin. The isolates of the various farms showed identical or almost identical characteristics. The 79 group B streptococci were additionally investigated by macrorestriction analysis of their chromosomal DNA by using the restriction endonucleases SmaI, ApaI and SalI. The restriction patterns obtained by pulsed-field gel electrophoresis displayed identical or almost identical patterns for the cultures of the various farms, respectively, but different patterns for isolates among the various dairy farms. The pheno- and genotypic characteristics of the 79 group B streptococci of the present study revealed that a single S. agalactiae strain or at least closely related subtypes of this strain were responsible for the mastitis situation of the farms, respectively.

In the present investigation 101 streptococcal cultures isolated from dogs, cats, rabbits, guinea pigs, monkeys, horses, nutria, pigs, cattle and humans were identified and further characterized as Streptococcus agalactiae, belonging to Lancefield’s serological group B by using conventional and molecular methods. The isolated cultures were differentiated biochemically, serologically and by amplification of species-specific parts of the gene encoding the 16S ribosomal RNA, the 16S-23S rDNA intergenic spacer region and by amplification of the CAMP factor gene cfb by polymerase chain reaction (PCR).

The investigated group B streptococci were further characterized serologically for specific polysaccharide and protein antigens, partly supported by PCR-amplification of the respective genes.

The type antigen patterns mainly observed for group B streptococci isolated from dog, cat, rabbit, guinea pig, monkey, horse, nutria, pig and human origin were III/Rib, Ia and Ia/cα. The group B streptococci isolated from cattle mostly displayed protein antigen X, alone or in combination with polysaccharide antigen Ia. The group B streptococci, particularly those isolated from dogs and cats but also those isolated from rabbits, guinea pigs, monkeys, horses and humans, less pronounced for isolates from nutria and pigs were predominantly negative in the lactose reaction, orange-red pigmented, showed bacterial growth by formation of short chains and growth in fluid media with a uniform turbidity of the growth media and with diffuse colonies in soft agar. In addition, the isolates displayed a low surface hydrophobicity, an inability to hemagglutinate rabbit erythrocytes and were resistant to minocyclin and tetracycline. In contrast group B streptococci from cattle were lactose positive, weakly or not-pigmented, displayed bacterial growth with relatively long chain formation, growth in fluid media as sediment with clear supernatant and with compact colonies in soft agar. In addition the cattle isolates generally showed an increased surface hydrophobicity, a hemagglutination reaction with rabbit erythrocytes and were sensitive to minocyclin and tetracycline. Most of the group B streptococci of various origins were hyaluronidase positive demonstrated with a decapsulation test and had a hylB-gene amplicon with a size of

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[1.] Analyse:Kf/Fragment 130 01 - Diskussion
Bearbeitet: 4. October 2013, 22:02 Singulus
Erstellt: 17. May 2012, 13:28 (Anonym69)
Fragment, KeineWertung, Kf, SMWFragment, Schutzlevel, Unfertig, Yildirim 2002

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KeineWertung
Bearbeiter
Anonym69
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 130, Zeilen: 01
Quelle: Yildirim 2002
Seite(n): 135, Zeilen: 01
8. LITERATURVERZEICHNIS

ABDULMAWJOOD, A., WEIß, R., and LÄMMLER, CH., 1998: Species identification of Streptococcus porcinus by restriction fragment length polymorphism analysis of 16S ribosomal DNA. Res. Vet. Sci. 65: 85-86. ABDULMAWJOOD, A., and LÄMMLER, CH., 1999: Amplification of 16S ribosomal RNA gene sequences for the identification of streptococci of Lancefield group B. Res. Vet. Sci. 67: 159-162. ABDURAHMAN, O. A., AGAB, H., ABBAS, B., and ASTROM, G., 1995: Relations between udder infection and somatic cells in camel (Camelus dromedarius) milk. Acta Vet. Scand. 36: 423-431. ADDERSON, E. E., TAKAHASHI, S., and BOHNSACK, J. F., 2000: Bacterial genetics and human immunity to group B streptococci. Mol. Gen. Metabol. 71: 451-454. AHMET, Z., STANIER, P., HARVEY, D., and HOLT, D., 1999: New PCR primers for the sensitive detection and specific identification of group B betahemolytic streptococci in cerebrospinal fluid. Mol. Cell. Probes 13: 349-357. AKINEDEN, Ö., HASSAN, A. A., ANNEMÜLLER, C., LÄMMLER, CH., WOLTER, W. und ZSCHÖCK, M., 2000: Molekulare Untersuchung zum Vorkommen von Virulenzfaktoren bei Staphylococcus aureus-Kulturen, isoliert von subklinischen Rindermastitiden. 41. Arbeitstagung des Arbeitsgebietes Lebensmittelhygiene vom 25.09.-28.09.2000 in Garmisch-Partenkirchen. Tagungsbericht: 703-708. AMBORSKI, R. L., SNIDER III, T. G., THUNE, R. L., and CULLER, D. D., 1983: A non-hemolytic, group B streptococcus infection in cultured bullfrogs, Rana catesbeiana, in Brazil. J. Wildlife Dis. 19: 180-184.

9. Literaturverzeichnis

ABDULMAWJOOD, A., WEIß, R., und LÄMMLER, CH., 1998: Species identification of Streptococcus porcinus by restriction fragment length polymorphism analysis of 16S ribosomal DNA. Res. Vet. Sci. 65: 85-86 ABDULMAWJOOD, A., und LÄMMLER, CH., 1999: Amplification of 16S ribosomal RNA gene sequences for the identification of streptococci of Lancefield group B. Res. Vet. Sci. 67: 159-162 ABRAHAM, S. N., BEACHEY, E. H., und SIMPSON, W. A., 1983: Adherence of Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa to fibronectin-coated and uncoated epithelial cells. Infect. Immun. 41: 1261-1268 ADDERSON, E. E., TAKAHASHI, S., und BOHNSACK, J.F., 2000: Bacterial genetics and human immunity to group B streptococci. Mol. Genet. Metab. 71: 451-454 AHMET, Z., STANIER, P., HARVEY, D., und HOLT, D., 1999: New PCR primers for the sensitive detection and specific identification of group B beta-hemolytic streptococci in cerebrospinal fluid. Mol. Cell. Probes. 13 : 349-357 AMBORSKI, R. L., SNIDER III, T. G., THUNE, R. L., und CULLER, D. D. 1983: A non-hemolytic, group B Streptococcus infection in cultured Bullfrogs, Rana catesbeiana, in Brazil. J. Wildlife Dis. 19: 180-184

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Sichter

[2.] Analyse:Kf/Fragment 130 34 - Diskussion
Bearbeitet: 4. October 2013, 22:04 Singulus
Erstellt: 17. May 2012, 14:59 (Anonym69)
Fragment, KeineWertung, Kf, SMWFragment, Schutzlevel, Unfertig, Yildirim 2002

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KeineWertung
Bearbeiter
Ananym69
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 130, Zeilen: 34-51
Quelle: Yildirim 2002
Seite(n): 136, Zeilen: 01-36
BAKER, C. J., and BARETT, F. F., 1974:

Group B streptococcal infections in infants. The importance of the various serotypes. JAMA 230: 1158-1160. BAKER, C. J., KASPER, D. L., and DAVID, C. E., 1976: Immunochemical characterization of the "native" type III polysaccharide of group B Streptococcus. J. Exp. Med. 143: 258-270. BAKER, C. J., 1980: Group B streptococcal infections. In: Stollerman, G., H., (ed.) Adv. Intern. Med. 25: 475-501. BAKER, C. J., 1990: Immunization to prevent group B streptococcal disease: victories and vexations. J. Infect. Dis. 161: 917-921. BAKER, C. J., 1997: Group B streptococcal infections. Clin. Perinatol. 24: 59-70.

BAKER, C. J., und BARETT, F. F., 1974:

Group B streptococcal infections in infants. The importance of the various serotypes. J. Am. Med. Assoc. 230: 1158-1160 BAKER, C. J., KASPER, D. L., und DAVID, C. E., 1976: Immunochemical characterization of the "native" type III polysaccharide of group B Streptococcus. J. Exp. Med. 143: 258-270 BAKER, C. J., und KASPER, D. L., 1976a: Correlation of maternal antibody deficiency with susceptibility to neonatal group B streptococcal infection. N. Engl.J. Med. 294: 753-756 BAKER, C. J., EDWARDS, M. S., und KAPSER, D. L., 1978: Immunogenicity of polysaccharides from type III group B Streptococcus. J. Clin. Invest. 61: 1107-1110 BAKER, C. J., 1980: Group B streptococcal infections. In: STOLLERMAN, G, H., (Hrsg.): Advences in Internal Medicine. Year Book Medical Publishers Inc., Chicago, USA. 25: 475-501 BAKER, C. J., KASPER, D. L., WEBB, B. J., und EDWARDS, M. S., 1986: The role of complement and antibody in opsonophagocytosis of type II group B streptococci. J. Infect. Dis. 154: 47-54 BAKER, C. J., 1990: Immunization to prevent group B streptococcal disease: victories and vexations. J. Infect. Dis. 161: 917-921 BAKER, C. J., und EDWARDS, M. S., 1995: Group B streptococcal infections. In: REMINGTON, J. S., und KLEIN, J. O., (4th Hrsg.) Infectious diseases of the fetus and newborn infant. Lee and Philadelphia, USA. BAKER, C. J., 1997: Group B streptococcal infections. Clin. Perinatol. 24: 59-70

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Quelle Autor Titel Verlag Jahr Lit.-V. FN
Kf/Yildirim 2002 Ali Önder Yildirim Phäno- und Genotypisierung von Streptokokken der serologischen Gruppe B (Streptococcus agalactiae), isoliert von verschiedenen Tierarten 2002 ja ja


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