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Typus
BauernOpfer
Bearbeiter
Graf Isolan
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Untersuchte Arbeit:
Seite: 27, Zeilen: 16-28
Quelle: Shimizu et al 2003
Seite(n): 2310, Zeilen: li.Sp. 50-53 - re.Sp. 1-14
The cell sheet engineering process used the temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) to coat tissue culture polystyrene (TCPS) dishes, changing their property from hydrophobic to hydrophilic with change of temperature 302. Normal TCPS dishes are hydrophobic and absorb extracellular matrix (ECM) proteins resulting in cell attachment and proliferation. The surfaces of PIPAAm are hydrophobic and cells adhere and proliferate under culture condition at 37° C. By lowering temperature below 32° C, the surfaces change reversibly to hydrophilic and not cell adhesive due to rapid hydration and swelling of the grafted PIPAAm. This unique surface change allows cultured cells to detach spontaneously from these grafted surfaces simply by lowering temperature. However, unlike trypsin treatment to harvest cells from culture dishes (which cause disruption to both adhesive proteins and membrane receptors and cells detach with considerable damages and cells are separated), this method allows harvesting cells in a single layer. These are then piled up to form a multilayer tissue like structure.

302. Shimizu T, Yamato M, Kikuchi A, Okano T (2003) Cell sheet engineering for myocardial tissue reconstruction. Biomaterials 24 (13):2309-16

Normal tissue culture polystyrene (TCPS) dishes are hydrophobic and absorb ECM proteins resulting in cell attachment and proliferation. To harvest cells from the surfaces, enzymatic digestion including trypsin and dispase are usually utilized. In that case, both adhesive proteins and membrane receptors are disrupted, then cells detach with considerable damages (Fig. 2B). On the other hand, we graft temperature-responsive polymer, poly(N-isopropylacrylamide)(PIPAAm) to TCPS dishes covalently by electron beam. The surfaces are hydrophobic and cells adhere and proliferate under culture condition at 37°C. By lowering temperature below 32°C, the surfaces change reversibly to hydrophilic and not cell adhesive due to rapid hydration and swelling of the grafted PIPAAm. This unique surface change allows cultured cells to detach spontaneously from these grafted surfaces simply by lowering temperature [18].

[18] Yamada N, Okano T, Sakai H, Karikusa F, Sawasaki Y, Sakurai Y. Thermo-responsive polymeric surfaces; control of attachment and detachment of cultured cells. Makromol Chem Rapid Commun 1990;11:571–6.

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