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Graf Isolan
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Untersuchte Arbeit:
Seite: 28, Zeilen: 3-4
Quelle: Ott et al 2008
Seite(n): 1 (Internetversion), Zeilen: re. Sp. 1ff
However, for other tissue like heart, it is still in the laboratorywith no promising results 51, 68, 101, 103, 155, 244, 278.

51. Chen RN, Ho HO, Tsai YT, Sheu MT (2004) Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials 25 (13):2679-86

68. Dellgren G, Eriksson MJ, Brodin LA, Radegran K (2002) Eleven years' experience with the Biocor stentless aortic bioprosthesis: clinical and hemodynamic follow-up with long-term relative survival rate. Eur J Cardiothorac Surg 22 (6):912-21

101. Gerecht-Nir S, Radisic M, Park H, Cannizzaro C, Boublik J, Langer R, Vunjak-Novakovic G (2006) Biophysical regulation during cardiac development and application to tissue engineering. Int J Dev Biol 50 (2-3):233-43

103. Gilbert TW, Sellaro TL, Badylak SF (2006) Decellularization of tissues and organs. Biomaterials 27 (19):3675-83

155. Ketchedjian A, Jones AL, Krueger P, Robinson E, Crouch K, Wolfinbarger L, Jr., Hopkins R (2005) Recellularization of decellularized allograft scaffolds in ovine great vessel reconstructions. Ann Thorac Surg 79 (3):888-96

244. Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA (2008) Perfusiondecellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 14 (2):213-21

278. Rieder E, Kasimir MT, Silberhumer G, Seebacher G, Wolner E, Simon P, Weigel G (2004) Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells. J Thorac Cardiovasc Surg 127 (2):399-405

To create a whole-heart scaffold with intact three-dimensional geometry and vasculature, we attempted to decellularize cadaveric hearts by coronary perfusion with detergents, which have been shown to generate acellular scaffolds for less complex tissues, by direct immersion10–14. We then repopulated decellularized rat hearts with neonatal cardiac cells or rat aortic endothelial cells and cultured these recellularized constructs under simulated physiological conditions for organ maturation15.

10. Dellgren, G., Eriksson, M.J., Brodin, L.A. & Radegran, K. Eleven years’ experience with the Biocor stentless aortic bioprosthesis: clinical and hemodynamic follow-up with long-term relative survival rate. Eur. J. Cardiothorac. Surg. 22, 912–921 (2002).

11. Rieder, E. et al. Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells. J. Thorac. Cardiovasc. Surg. 127, 399–405 (2004).

12. Ketchedjian, A. et al. Recellularization of decellularized allograft scaffolds in ovine great vessel reconstructions. Ann. Thorac. Surg. 79, 888–896 (2005).

13. Chen, R.N., Ho, H.O., Tsai, Y.T. & Sheu, M.T. Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials 25, 2679–2686 (2004).

14. Gilbert, T.W., Sellaro, T.L. & Badylak, S.F. Decellularization of tissues and organs. Biomaterials 27, 3675–3683 (2006).

15. Gerecht-Nir, S. et al. Biophysical regulation during cardiac development and application to tissue engineering. Int. J. Dev. Biol. 50, 233–243 (2006).

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