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Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 56, Zeilen: 1-15
Quelle: Ruffins et al 2002
Seite(n): 581, Zeilen: re. Sp. 20-37, 49-52
Laser scanning microscopy

Laser scanning microscopy has continued to develop and has proved itself to be an excellent tool for in vivo microscopy due to its ability to collect ‘optical sections’ through the specimen. By raster scanning a laser beam over the focal plane and blocking light from elsewhere with a confocal pinhole in front of the detector, an image largely devoid of out-of-focus fluorescence can be generated. Confocal Laser Scanning Microscopy (CLSM) works well for imaging near the surface (within 100 μm). At greater depths, scattering in the tissue and blurring due to the tissue optics make collection of light through a detector pinhole inefficient. A more significant limitation is the photo bleaching from CLSM, because it excites fluorescence throughout the depth of the specimen even when it is collecting a single optical section. Despite these difficulties, CLSM images have offered important glimpses into the developing embryo 279, 374. In preparations with no absorbance of the intense infrared pulses and limited light scattering, two-photon microscopy has permitted observation of live cells and intact tissues with startling resolution 238, 262.


238. Nimchinsky EA, Oberlander AM, Svoboda K (2001) Abnormal development of dendritic spines in FMR1 knock-out mice. J Neurosci 21 (14):5139-46

262. Potter SM, Pine J, Fraser SE (1996) Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy. Scanning Microsc Suppl 10 189-99

279. Ritter DA, Bhatt DH, Fetcho JR (2001) In vivo imaging of zebrafish reveals differences in the spinal networks for escape and swimming movements. J Neurosci 21 (22):8956-65

374. Wallingford JB, Ewald AJ, Harland RM, Fraser SE (2001) Calcium signaling during convergent extension in Xenopus. Curr Biol 11 (9):652-61

Laser scanning microscopy

Laser scanning microscopy has continued to develop and has proved itself to be an excellent tool for in vivo microscopy due to its ability to collect ‘optical sections’ through the specimen. By raster scanning a laser beam over the focal plane and blocking light from elsewhere with a confocal pinhole in front of the detector, an image largely devoid of out-of-focus fluorescence can be generated. Confocal laser scanning microscopy (CLSM) works well for imaging near the surface (within 100 μm). At greater depths, scattering in the tissue and blurring due to the tissue optics make collection of light through a detector pinhole inefficient. A more significant limitation is the photobleaching from CLSM, because it excites fluorescence throughout the depth of the specimen even when it is collecting a single optical section. Despite these difficulties, CLSM images have offered important glimpses into the developing embryo [20,21].

[...] In preparations with no absorbance of the intense infrared pulses and limited light scattering, two-photon microscopy has permitted observation of live cells and intact tissues with startling resolution [23–25].


20. Wallingford JB, Ewald AJ, Harland RM, Fraser SE: Calcium signaling during convergent extension in Xenopus. Curr Biol 2001, 11:652-661.

21. Ritter DA, Bhatt DH, Fetcho JR: In vivo imaging of zebrafish reveals differences in the spinal networks for escape and swimming movements. J Neurosci 2001, 21:8956-8965.

23. Potter SM, Pine J, Fraser SE: Neural transplant staining with DiI and vital imaging with 2-photon laser-scanning microscopy. Scanning Microsc Suppl 1996, 10:189-199.

24. Nimchinsky EA, Oberlander AM, Svoboda K: Abnormal development of dendritic spines in fMR1 knock-out mice. J Neurosci 2001, 21:5139-5146.

25. Kaneko T, Fujita K, Tanaka H, Oyamada M, Nakamura O, Kawata S, Takamatsu T: Real-time two-photon microscopy and its application for in situ imaging. Acta Histochem 2001, 34:399-403.

Anmerkungen

Identisch bis hin zu den Literaturverweisen. Ohne Hinweis auf eine Übernahme.

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