Fandom

VroniPlag Wiki

Analyse:Sih/Fragment 175 17

31.357Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.


Typus
Verschleierung
Bearbeiter
Graf Isolan
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 175, Zeilen: 17-22, 24-29
Quelle: Giaever und Keese 1983
Seite(n): 219, 221, Zeilen: 219:li.Sp. 43-49 - re.Sp. 1-3; 221:re.Sp. 4-7
This method of cell culture has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces, which, in general, have polar molecular inhomogeneities. In addition, cells grown on such substrates can be transferred by simply pipetting the cell layer. Such procedure of cell harvesting is especially important where the effect of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells can be avoided. It is here to be mentioned that the compositions of cell membrane proteins are changed by enzymatic treatment or by mechanical scraping 301. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments. If the adsorbed serum proteins at the interface are crosslinked by glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat 88, the patterns of cell growth can be altered significantly.

88. Feder J, Giaever I (1980) Adsorption of ferritin. J Colloid Interface Sci 78 144-54

301. Shaw DJ (1966) Introduction to Collid and Surface Chemistry. Butterworths, London

[Seite 219]

It has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces which, in general, have polar molecular inhomogeneities (3, 5). In addition, cells grown on such substrates can be transferred from one fluid interface to another or from a fluid to a solid substrate by simply pipetting the cell layer. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments.

[Seite 221]

If the adsorbed serum proteins at the interface. are crosslinked by using glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat (6), the patterns of cell growth can be altered significantly.


3. Rosenberg, M. D. (1965) in Baroda Tissue Culture Seminar, ed. Ramakrishnan, C. V. (Junk, The Hague, The Netherlands), pp. 93-107.

4. Shaw, D. J. (1966) Introduction to Collid and Surface Chemistry (Butterworths, London), p. 57.

5. Maroudas, N. G. (1973) Nature (London) 244, 353-354.

6. Feder, J. & Giaever, I. (1980) J. Colloid Interface Sci. 78, 144-154.

Anmerkungen

Ohne Hinweis auf eine Übernahme. Die Formulierungen der Vorlage werden an dieser Stelle gleich zweimal verwendet. Dasselbe ist schon mal auf Seite 42 passiert (vgl. Sih/Fragment_042_01).

Sichter
(Graf Isolan)

Auch bei Fandom

Zufälliges Wiki