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Acute effects of garlic extract on spreading depression and synaptic activity in rat brain slices

von Dr. Claudia Marschollek

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[1.] Clm/Fragment 021 01 - Diskussion
Zuletzt bearbeitet: 2014-05-09 18:36:45 Singulus
Clm, Fragment, Gesichtet, Haarmann 2009, SMWFragment, Schutzlevel sysop, Verschleierung

Typus
Verschleierung
Bearbeiter
Hindemith
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 21, Zeilen: 1ff (komplett)
Quelle: Haarmann 2009
Seite(n): 13, 14, Zeilen: 13: last lines; 14: 1-21
[SD duration was defined] as the interval between the time of half-maximal voltage shift during onset and recovery of the negative DC potential deflection.

Long-term potentiation

Single pulses of electrical stimulation were applied through a bipolar platinum electrode attached to the white matter perpendicular to the recording electrodes. Evoked field excitatory postsynaptic potentials (fEPSP) were recorded in the third layer of neocortical slices. The fEPSP was elicited by adjusting the intensity of stimulation to 50% of that at which population spikes after fEPSP began to appear. The amplitude of fEPSP 1 ms after the onset was measured for data analysis. In long-term potentiation (LTP) experiments, the cortex was sequentially stimulated once every minute. Ten trains of four pulses (pulse duration 0.1 msec; interpulse interval 50 msec; intensity 5 V) were repeated at intervals of 10 msec. LTP was operationally defined as the mean change in fEPSP amplitude in response to five stimuli given 30 min after tetanic stimulation compared with the mean response to five test pulses applied immediately before the stimulation. Thus % potentiation = [(posttetanus amplitude of fEPSP/baseline amplitude of fEPSP) 1] 100. Tetanic stimulation was applied 60 min after application of drug.

Experimental protocols

The experimental protocol consisted of four periods as follows: (a) control period, neocortical slices were superfused with ACSF (30 min), tested for spontaneous CSD; (b) KCl injection, induction of SD (CSD1); (c) application of garlic oil (1-500 μl/l) before the second injection of KCl (CSD2); (d) washout of garlic oil with ASCF (45 min, second control period), third injection of KCl (CSD3). Only a single concentration of garlic oil was used in a given slice.

SD duration was defined as the interval between the time of half-maximal voltage shift during onset and recovery of the negative DC potential deflection.

Long-term potentiation

[page 14]

Single pulses of electrical stimulation were applied through a bipolar platinum electrode attached to the white matter perpendicular to the recording electrodes. Evoked field excitatory postsynaptic potentials (fEPSP) were recorded in the third layer of neocortical slices. The fEPSP was elicited by adjusting the intensity of stimulation to 50% of that at which population spikes after fEPSP began to appear. The amplitude of fEPSP 1 ms after the onset was measured for data analysis. In long-term potentiation (LTP) experiments, the cortex was sequentially stimulated once every minute. Ten trains of four pulses (pulse duration 0.1 msec; interpulse interval 50 msec; intensity 5 V) were repeated at intervals of 10 msec. LTP was operationally defined as the mean change in fEPSP amplitude in response to five stimuli given 30 min after tetanic stimulation compared with the mean response to five test pulses applied immediately before the stimulation. Thus % potentiation = [(posttetanus amplitude of fEPSP/baseline amplitude of fEPSP) 1] 100. Tetanic stimulation was applied 60 min after application of drug.

Experimental protocols

The experimental protocol consisted of four periods as follows: (a) control period, neocortical slices were superfused with ACSF (30 min), tested for spontaneous SD; (b) KCl injection, induction of SD (SD1); (c) application of D2 dopamine receptor agonist quinpirole (10-200 μM), or the dopamine D2 dopamine receptor antagonist sulpiride (0.1-10 μM, 60 min) before the second injection of KCl (SD2); (d) washout of quinpirole or sulpiride with ASCF (45 min, second control period), third injection of KCl (SD3). Only a single concentration of quinpirole or sulpiride was used in a given slice

Anmerkungen

The source is not given.

Sichter
(Hindemith) Agrippina1


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