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Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 20, Zeilen: 1ff (komplett)
Quelle: Haarmann 2009
Seite(n): 13, Zeilen: 3ff
Material and methods

The experiments were performed on adult rat (200-350g) somatosensory neocortical slices. The brain was removed under deep methohexital anaesthesia and placed in cold (1–4°C) artificial cerebrospinal fluid (ACSF) pre-equilibrated with 5% CO2 in O2 to give a pH of 7.4. The ACSF contained (in mM): NaCl 124, KCl 4, CaCl2 1.0, NaH2PO4 1.24, MgSO4 1.3, NaHCO3 26 and glucose 10. The somatosensory neocortices were dissected and cut into slices of 500 μm thickness. The slices were incubated in ACSF solution for >1 h at 28°C. After 30-min incubation, CaCl2 was elevated to 2.0 mM. Slices were transferred to an interphase-type experimental chamber and superfused with ACSF at 32°C (1.5–2 ml/min).

Electrophysiological recordings

Extracellular field potentials were recorded with glass microelectrodes (150 mmol/l NaCl; 2–10 MΩ) connected to the amplifier by an Ag/AgCl–KCl bridge in the third and the fifth layers of neocortical tissues. Field potentials were traced by an ink-writer and recorded by a digital oscilloscope.

Induction of neocortical SD

SD was elicited by KCl microinjection. A glass electrode filled with 2 M KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the sixth layer of the neocortical slices. A high-pressure pulse was applied to inject an amount of K+ in the tissue sufficient to induce cortical SD (tip diameter: 2 μm; injection pressure 0.5–1.0 bar applied for 200–300 ms, two injections, 1–3 nl per pulse). Cortical SD-like events were evaluated with respect to their amplitude, duration and velocity rates. SD duration was defined [as the interval between the time of half-maximal voltage shift during onset and recovery of the negative DC potential deflection.]

Material and methods

The experiments were performed on adult rat (250-350g) somatosensory neocortical slices. The brain was removed under deep methohexital anaesthesia and placed in cold (1–4°C) artificial cerebrospinal fluid (ACSF) pre-equilibrated with 5% CO2 in O2 to give a pH of 7.4. The ACSF contained (in mM): NaCl 124, KCl 4, CaCl2 1.0, NaH2PO4 1.24, MgSO4 1.3, NaHCO3 26 and glucose 10. The somatosensory neocortices were dissected and cut into slices of 500 μm thickness. The slices were incubated in ACSF solution for >1 h at 28°C. After 30-min incubation, CaCl2 was elevated to 2.0 mM. Slices were transferred to an interphase-type experimental chamber and superfused with ACSF at 32°C (1.5–2 ml/min).

Electrophysiological recordings

Extracellular field potentials were recorded with glass microelectrodes (150 mmol/l NaCl; 2–10 M ) connected to the amplifier by an Ag/AgCl–KCl bridge in the third and the fifth layers of neocortical tissues. Field potentials were traced by an ink-writer and recorded by a digital oscilloscope.

Induction of neocortical SD

SD was elicited by KCl microinjection. A glass electrode filled with 2 M KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the sixth layer of the neocortical slices. A high-pressure pulse was applied to inject an amount of K+ in the tissue sufficient to induce cortical SD (tip diameter: 2 μm; injection pressure 0.5–1.0 bar applied for 200–300 ms, two injections, 1–3 nl per pulse). Cortical SD-like events were evaluated with respect to their amplitude, duration and velocity rates. SD duration was defined as the interval between the time of half-maximal voltage shift during onset and recovery of the negative DC potential deflection.

Anmerkungen

Though identical, nothing has been marked as a citation.

Sichter
(Graf Isolan) Schumann

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