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2 ungesichtete Fragmente: "verdächtig" oder "Keine Wertung"

[1.] Dsa/Fragment 016 01 - Diskussion
Bearbeitet: 10. August 2016, 07:26 (WiseWoman)
Erstellt: 17. May 2015, 16:56 Hindemith
Celley 2003, Dsa, Fragment, KeineWertung, SMWFragment, Schutzlevel, ZuSichten

Typus
KeineWertung
Bearbeiter
Hindemith
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 16, Zeilen: 1-3
Quelle: Celley 2003
Seite(n): 1 (online source), Zeilen: -
Every 24 hours, the kidneys filter and clean about 200 quarts of fluid from the blood. Of this, about 198 quarts are absorbed back and retained in the body, and about two quarts are sent to the bladder in the form of urine. Every 24 hours, the kidneys filter and clean about 200 quarts of fluid from the blood. Of this, about 198 quarts are absorbed back and retained in the body, and about two quarts are sent to the bladder in the form of urine.

Source: http://www.kidney.org

Anmerkungen

No source is given.

It is surprising that the non-SI unit "quart" makes it into a scientific publication.

Apparently the text originates from the website www.kidney.org . There however, it cannot be located anymore.

Sichter
(Hindemith)

[2.] Dsa/Fragment 055 05 - Diskussion
Bearbeitet: 30. May 2015, 10:24 (Kybot)
Erstellt: 18. May 2015, 16:17 Hindemith
Dsa, Fragment, KeineWertung, SMWFragment, Schutzlevel, Wulff et al 2002, ZuSichten

Typus
KeineWertung
Bearbeiter
Hindemith
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 55, Zeilen: 5-16
Quelle: Wulff et al 2002
Seite(n): 1264, Zeilen: l.col: 1ff
In brief, a conditional targeting vector was generated from a 7-kb fragment encompassing the entire transcribed region on 12 exons. The neomycin resistance cassette was flanked by two loxP sites and inserted into intron 11. Exons 4–11, which code for the sgk1 kinase domain, were “floxed” by inserting a third loxP site into intron 3. A clone with a recombination between the first and third loxP site (type I recombination) was injected into C57BL/6 blastocytes. Male chimeras were bred to C57BL/6 and 129/SvJ females. Heterozygous SGK1-deficient mice were backcrossed to 129/SvJ wild-type mice (Charles River, Sulzfeld, Germany) for ten generations and then intercrossed to generate homozygous SGK1 knockout mice (sgk1−/−) and their wild type littermates (sgk1+/+). Male and female sgk1−/− mice were used in the studies and compared with littermate sgk1+/+ mice of the respective gender. Mice were genotyped by PCR (Huang Y. et al., (2004) J Am Soc Nephol; Wulff P. et al., (2002) J Clin Invest). A 7-kb fragment encompassing the entire transcribed region on 12 exons was subcloned into pBluescript (Stratagene, La Jolla, California, USA). This plasmid was used for generation of a conditional targeting vector (Figure 1a). The vector was linearized at a unique NotI site and electroporated into R1 ES cells. Positive clones were identified by Southern blot analysis. A targeted ES cell clone was transiently transfected with Cre recombinase. The resulting recombination types were identified by Southern blot analysis. One clone with a recombination between the first and third loxP site (type I recombination) was injected into C57BL/6 blastocytes. Male chimeras were bred to C57BL/6 and 129/SvJ females. Heterozygous sgk1-deficient mice were either inbred or backcrossed to 129/SvJ wild-type mice for two generations and then intercrossed to generate homozygous sgk1–/– and sgk1+/+ mice. Male and female sgk1–/– mice were used in the studies and compared with littermate sgk1+/+ mice of the respective gender. Mice were genotyped by PCR.
Anmerkungen

The source is given before the documented passage as well as at the end of it, but it is not clear that the description of the method is taken from it mostly verbatim.

Sichter
(Hindemith)

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