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[1.] Iam/Fragment 036 01 - Diskussion
Zuletzt bearbeitet: 2014-03-12 20:03:32 Graf Isolan
Fragment, Gesichtet, Iam, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Sheikh 2006

Typus
KomplettPlagiat
Bearbeiter
Graf Isolan, Hindemith
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 36, Zeilen: 1-10
Quelle: Sheikh 2006
Seite(n): 30-31, Zeilen: 30:1-9 - 31:1-2
3.2.2.b. Thermal cycler amplification program

The amplification was performed at 50 °C for 2 min, 95°C for 2 min., 95°C for 15 seconds to 60°C for 30 seconds for 45 cycles (Figure 8) in an ABI prism 7000 sequence detection system. All samples were assayed in duplicate. Expression of different genes was analyzed using Platinum SYBR Green qPCR mix UDG. The PCR amplification program was followed by dissociation curve protocol for controlling the specificity of the PCR products. Specific temperature of dissociation of the PCR product was calculated by the Primer Express software. Curves of amplification were analyzed to measure the Ct value in the linear range of the amplification. The results were normalized to the house keeping gene and fold change expression was calculated using Ct values by Prism Graph Pad 4 software.

36a diss Iam.png

Figure 8: Thermal cycler amplification program for the quantitative real-time PCR amplification of the mRNA using Platinum® SYBR® Green qPCR SuperMix UDG, specific forward, reverse primer and template cDNA in ABI Prism® 7000 Sequence Detection System by Applied Biosystems. Stage 1; 2 minutes incubation at 50°C, Stage 2; 2 minutes incubation at 95°C for hot start, Stage 3; 15 sec at 95°c and 30sec at 60°C for 45 repeats, Stage 4; 15 sec 95°C, 15sec 60°C and 15 sec 95°C to get dissociation curve.

[Page 30]

3.2.1.II Thermal cycler amplification program

The amplification was performed at 50 °C for 2 min., 95°C for 2 min., 95°C for 15 sec to 60°C for 30 sec for 45 cycles (Figure 2) in an ABI prism 7000 sequence detection system. All samples were assayed in duplicate. Expression of different genes was analysed using Platinum SYBR Green qPCR mix UDG. The PCR amplification program was followed by dissociation curve protocol for controlling the specificity of the PCR products. Specific temperature of dissociation of the PCR product was calculated by the Primer Express software. Curves of amplification were analysed to measure the Ct value in the linear range of the amplification. The results were normalized to the

[Page 31]

housekeeping gene and fold change expression was calculated using Ct values by Prism Graph Pad 4 software.

36a source Iam.png

Figure 3: Thermal cycler amplification program for the quantitative real-time PCR amplification of the mRNA using Platinum® SYBR® Green qPCR SuperMix UDG, specific forward, reverse primer pairs and template cDNA in ABI Prism® 7000 Sequence Detection System by Applied Biosystems. Stage 1; 2 min. incubation at 50°C, Stage 2; 2 min. incubation at 95°C for hot start, Stage 3; 15 sec at 95°C and 30 sec at 60°C for 45 repeats. Stage 4; 15 sec at 95°C; 15 sec at 60°C and 15 sec at 95°C to get dissociation curve.

Anmerkungen

But for some "corrections" identical up to the breaks between words ("50 °C" vs. "95°C" in both texts), though nothing has been marked as a citation. Iam's "figure 8" is also identical to Sheiks "figure 3"

Sichter
(Graf Isolan) Schumann


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