Fandom

VroniPlag Wiki

Iam/045

< Iam

31.377Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Teilen

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.

Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
[1.] Iam/Fragment 045 01 - Diskussion
Zuletzt bearbeitet: 2014-03-12 20:16:45 Graf Isolan
Fragment, Gesichtet, Iam, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Zibrova 2004

Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 45, Zeilen: 1-12
Quelle: Zibrova 2004
Seite(n): 64, Zeilen: 5-17
3.3.2.d Immunovisualization

After transfer, the membrane was incubated on the rocking platform with blocking solution overnight at 4°C. Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 2 h at room temperature. After washing (six times, five min on each occasion), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 h at room temperature. Afterwards, the membrane was washed as before. For the chemiluminescent detection SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 min at room temperature, laid between two sheets of transparent plastic protector and exposed to X-ray film, which was developed afterward according to the manufacturer’s instructions.

Immunovisualization

After transfer, the membrane was incubated on a rocking platform with blocking solution overnight at 4°C (or alternatively, for 60 minutes at room temperature). Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 1 hour at room temperature. After washing (six times five minutes each), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 hour at room temperature. Afterwards the membrane was washed as before. For the chemiluminescent detection, SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 minutes at room temperature, laid between sheets of transparent plastic protector and exposed to X-ray film, which was developed afterwards according to manufacturer’s instructions.

Anmerkungen

Nearly identical but for "corrections" (and a minimal adaption). Nevertheless no part has been marked as a citation.

Sichter
(Graf Isolan) Schumann


vorherige Seite | zur Übersichtsseite | folgende Seite
Letzte Bearbeitung dieser Seite: durch Benutzer:Graf Isolan, Zeitstempel: 20140312202043

Auch bei Fandom

Zufälliges Wiki