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Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 45, Zeilen: 1-12
Quelle: Zibrova 2004
Seite(n): 64, Zeilen: 5-17
3.3.2.d Immunovisualization

After transfer, the membrane was incubated on the rocking platform with blocking solution overnight at 4°C. Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 2 h at room temperature. After washing (six times, five min on each occasion), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 h at room temperature. Afterwards, the membrane was washed as before. For the chemiluminescent detection SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 min at room temperature, laid between two sheets of transparent plastic protector and exposed to X-ray film, which was developed afterward according to the manufacturer’s instructions.

Immunovisualization

After transfer, the membrane was incubated on a rocking platform with blocking solution overnight at 4°C (or alternatively, for 60 minutes at room temperature). Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 1 hour at room temperature. After washing (six times five minutes each), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 hour at room temperature. Afterwards the membrane was washed as before. For the chemiluminescent detection, SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 minutes at room temperature, laid between sheets of transparent plastic protector and exposed to X-ray film, which was developed afterwards according to manufacturer’s instructions.

Anmerkungen

Nearly identical but for "corrections" (and a minimal adaption). Nevertheless no part has been marked as a citation.

Sichter
(Graf Isolan) Schumann

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