Fandom

VroniPlag Wiki

Mmu/Fragment 008 01

< Mmu

31.377Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Teilen

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.


Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 8, Zeilen: 1-18
Quelle: Shinawi and Cheung 2008
Seite(n): 761-762, 763, Zeilen: 761:right col. 5-8.11 - 762:left col. 1.4-10; Figure 2: legend 6-7; 763:left col. 6-11, right col. 10-12
The higher resolution and throughput with possibilities for automation, robustness, simplicity, high reproducibility and precise mapping of aberrations are the most significant advantages of aCGH over cytogenetic methods. In addition, there is no need for cell culture, making the turn around time shorter than in cytogenetic methods. As with other clinical diagnostic methods, there are limitations in aCGH technology. aCGH is not able to identify balanced rearrangements such as translocations and inversions and low-level mosaicism for unbalanced numeric or structural rearrangements.

1.2 Array – CGH Methodologies

In aCGH, equal amounts of labelled genomic DNA from a test and a reference sample are co-hybridized to an array containing the DNA targets. Genomic DNA of the patient and control are differentially labelled with Cyanine 3 (Cy3) and Cyanine 5 (Cy5). The slides are scanned into image files using a microarray scanner. The spot intensities are measured and the image files are quantified using feature extraction software, and text file outputs from the quantitative analyses are imported into software programs for copy number analysis (Fig.2) (Cheung 2005, Lu 2007). The resulting ratio of the fluorescence intensities is proportional to the ratio of the copy numbers of DNA sequences in the test and reference genomes.


17. Cheung S.W. et al. (2005) Development and validation of a CGH microarray for clinical cytogenetic diagnosis. Genet. Med. 7, 422–432

43. Lu, X. et al. (2007) Clinical implementation of chromosomal microarray analysis: summary of 2513 postnatal cases. PLoS ONE 2, e327

[Page 761]

aCGH methodology

In aCGH, equal amounts of labeled genomic DNA from a test and a reference sample are cohybridized to an array containing the DNA targets. [...] Genomic DNA of the patient and control are differen-

[Page 762]

tially labeled with Cyanine 3 (Cy3) and Cyanine 5 (Cy5) (Fig. 2a). [...] The spot intensities are measured (Fig. 2c) and the image files are quantified using feature extraction software, and text file outputs from the quantitative analyses are imported into software programs for copy number analysis (Fig. 2d) [4,9]. The resulting ratio of the fluorescence intensities is proportional to the ratio of the copy numbers of DNA sequences in the test and reference genomes.


FIGURE 2

[...] (b) The slides are scanned into image files using a specific microarray scanner.

[Page 763]

The advantages and limitations of diagnostic aCGH

The higher resolution and throughput with possibilities for automation, robustness, simplicity, high reproducibility and precise mapping of aberrations are the most significant advantages of aCGH over cytogenetic methods. In addition, there is no need for cell culture, making the turn around time shorter than in cytogenetic methods. [...]

As with other clinical diagnostic methods, there are limitations in aCGH technology. aCGH is not able to identify balanced rearrangements such as translocations and inversions.


4 Cheung, S.W. et al. (2005) Development and validation of a CGH microarray for clinical cytogenetic diagnosis. Genet. Med. 7, 422–432

9 Lu, X. et al. (2007) Clinical implementation of chromosomal microarray analysis: summary of 2513 postnatal cases. PLoS ONE 2, e327

Anmerkungen

Nothing has been marked as a citation. The source is not given.

Sichter
(Graf Isolan), SleepyHollow02

Auch bei Fandom

Zufälliges Wiki