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Functional characterization of the ‘PBX interacting protein’ (HPIP) in normal and malignant human haematopoiesis.

von Dr. Pak

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[1.] Pak/Fragment 013 01 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:28:42 Hindemith
Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia umbilical cord 2008

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Seite: 13, Zeilen: 1-2
Quelle: Wikipedia_umbilical_cord_2008
Seite(n): 1 (online source), Zeilen: -
[The blood within the umbilical cord, known as cord blood, is a rich and readily available source of primitive, undifferentiated stem] cells (of type CD34+ and CD38-). These cord blood cells can be used for bone marrow transplant. Recently, it has been discovered that the blood within the umbilical cord, known as cord blood, is a rich and readily available source of primitive, undifferentiated stem cells (i.e. CD34-positive and CD38-negative). These cord blood cells can be used for bone marrow transplant.
Anmerkungen

The source is not given.

Note that the internal links in the Wikipedia translate 1-to-1 into underlined words in the thesis.

Also the next few lines are taken from the wikipedia: Quelle:Pak/Wikipedia_embryonic_stem_cell_2008

Sichter
(Hindemith) Agrippina1

[2.] Pak/Fragment 013 02 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:29:16 Hindemith
Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia embryonic stem cell 2008

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Seite: 13, Zeilen: 2-4
Quelle: Wikipedia embryonic stem cell 2008
Seite(n): 1 (online source), Zeilen: -
Embryonic Stem (ES) cells are pluripotent stem cells. They are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. Embryonic Stem (ES) cells are pluripotent. This means they are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm.
Anmerkungen

The source is not mentioned.

Note that also the passage before is taken from the wikipedia: Quelle:Pak/Wikipedia_umbilical_cord_2008

Note that the internal links in the Wikipedia translate 1-to-1 into underlined words in the thesis.

Sichter
(Hindemith) Agrippina1

[3.] Pak/Fragment 013 17 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:36:23 Hindemith
Fragment, Gesichtet, Iwasaki and Akashi 2007, Pak, SMWFragment, Schutzlevel sysop, Verschleierung

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Untersuchte Arbeit:
Seite: 13, Zeilen: 17-27
Quelle: Iwasaki and Akashi 2007
Seite(n): 6692, Zeilen: l.col: 18-28, 33-41
The long-term reconstitution potential of human HSCs has been described in SCID mouse repopulating assays. In the most advanced xenotransplant models by utilizing the Rag2 and IL-2Rγ double-deficient mouse40 or the NOD/SCID IL2Rγ deficient mouse.41 The CD34+CD38- or the CD34+CD90+ fractions of human bone marrow and cord blood have been shown to reconstitute all human hematolymphoid lineage cells for a long term, indicating that these CD34+ fractions contains normal human HSCs. An injection of at least 1000 cells of the human CD34+CD38- bone marrow cells is required to obtain multi-lineage, longterm reconstitution in xenotransplant models. The percent of hCD34+CD38- or hCD34+CD90+ cells that are multipotent, long-term HSCs is still difficult to determine in a engraft model.

40. Traggiai E, Chicha L, Mazzucchelli L, et al. Development of a human adaptive immune system in cord blood cell-transplanted mice. Science. 2004;304:104-107.

41. Ishikawa F, Yasukawa M, Lyons B, et al. Development of functional human blood and immune systems in NOD/SCID/IL2 receptor {gamma} chain(null) mice. Blood. 2005;106:1565-1573.

The long-term reconstitution potential of human HSCs has been directly shown in SCID mouse repopulating assays. In the most advanced xenotransplant models by utilizing the Rag2 and IL-2Rγ double-deficient mouse (Traggiai et al., 2004) or the NOD–SCID IL2Rγ deficient mouse (Ishikawa et al., 2005), hCD34+hCD38- or the hCD34+hCD90+ fractions of human bone marrow and cord blood can reconstitute all human hematolymphoid lineage cells for a long term, indicating that these hCD34+ fractions contains normal human HSCs. [...] injection of at least 1000 cells of the human hCD34+hCD38- bone marrow cells is required to obtain multi-lineage, long-term reconstitution in xenotransplant models. Thus, it is unknown whether the HSC that is capable of reconstitution of all lineages at the single cell level exists in human hematopoiesis, and if so, what percent of hCD34+hCD38- or hCD34+hCD90+ cells are multipotent, longterm HSCs.

Traggiai E, Chicha L, Mazzucchelli L, Bronz L, Piffaretti JC, Lanzavecchia A et al. (2004). Development of a human adaptive immune system in cord blood cell-transplanted mice. Science 304: 104–107.

Ishikawa F, Yasukawa M, Lyons B, Yoshida S, Miyamoto T, Yoshimoto G et al. (2005). Development of functional human blood and immune systems in NOD/SCID/IL2 receptor {gamma} chain(null) mice. Blood 106: 1565–1573.

Anmerkungen

The source is not given.

Sichter
(Hindemith) Schumann


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