Fandom

VroniPlag Wiki

Pak/Fragment 033 01

< Pak

31.371Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Teilen

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.


Typus
Verschleierung
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 33, Zeilen: 1-3, 6-21
Quelle: Ahmed 2007
Seite(n): 33-34, Zeilen: 33: 1-2,14-18, 26-30 - 34: 1-8
[NOD/SCID mice were bred from the breeding] pairs originally obtained from Taconic Bomholt, Denmark and maintained in the animal facility located at the GSF-Haematology, Munich. All animals were handled under sterile conditions and maintained under micro isolators. [xenografts [sic] into immunodeficient NOD-SCID mice are the gold standard readout for human long-term repopulating hematopoietic cells.110-112]
3.1.5. Mice related reagents and equipment

Sterile Syringes: BD Plastipak 1 ml syringe (BD Biosciences, Palo Alto, CA) for injection of cells in mice and Kendall Monoject 3 ml syringes (Tyco Healthcare, UK) for bone marrow flushing and plating of CFC.

Sterile needles: 0.5 x 25 mm for intra venous injection of cells in mice and 0.55 x 25 mm (BD Microlance, Drogheda, Ireland) for bone marrow aspiration from living mice and flushing of bone marrow from extracted bones. 16 X 1.5 inch needles for dispensing and plating Methocult (CFC) media (Stem Cell Technologies, Vancouver, Canada).

Ammonium Chloride solution: For erythrocyte lysis 0.8% NH4Cl with 0.1 mM EDTA (Stem Cell Technologies, Vancouver, Canada).

Heparinized capillaries: (Microvette CB 300) plastic capillaries for collection of blood, containing 15 units (I.E) Lithium heparin per ml of blood (Sarstedt, Numbrecht, Germany).

3.1.6. Bacterial strain

• E. coli DH5á [sic]

3.1.5 Bacterial strain

• E. coli DH5α

[...]

NOD/SCID mice were bred from the breeding pairs originally obtained from Taconic Europe and maintained in the animal facility located at the GSF-Haematology, Munich. All animals were handled under sterile conditions and maintained under micro isolators.

3.1.7 Mice related reagents and equipment:

[...]

Sterile Syringes: BD Plastipak 1 ml syringe (BD Biosciences, Palo Alto, CA) for injection of cells in mice and Kendall Monoject 3 ml syringes (Tyco Healthcare, UK) for bone marrow flushing and plating of CFC.

Sterile needles: 0.5 x 25 mm for intra venous injection of cells in mice and 0.55 x 25 mm (BD Microlance, Drogheda, Ireland) for bone marrow aspiration from living

[page 34]

mice and flushing of bone marrow from extracted bones. 16 X 1.5 inch needles for dispensing and plating Methocult (CFC) media (Stem Cell Technologies, Vancouver, Canada)

Ammonium Chloride solution: For erythrocyte lysis 0.8% NH4Cl with 0.1 mM EDTA (Stem Cell Technologies, Vancouver, Canada)

Heparinized capillaries: (Microvette CB 300) plastic capillaries for collection of blood, containing 15 units (I.E) Lithium heparin per ml of blood (Sarstedt, Numbrecht, Germany).

Anmerkungen

The source is not given.

Sichter
(SleepyHollow02) Schumann

Auch bei Fandom

Zufälliges Wiki