Angaben zur Quelle [Bearbeiten]
| Autor | Petra Rocic, Christopher Kolz, Ryan Reed, Barry Potter, William M. Chilian |
| Titel | Optimal reactive oxygen species concentration and p38 MAP kinase are required for coronary collateral growth |
| Zeitschrift | American Journal of Physiology: Heart and Circulatory Physiology |
| Herausgeber | American Physiological Society |
| Ausgabe | 292 |
| Datum | 16. February 2007 |
| Seiten | H2729-H2736 |
| DOI | 10.1152/ajpheart.01330.2006 |
| URL | http://ajpheart.physiology.org/content/ajpheart/292/6/H2729.full.pdf |
Literaturverz. |
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| Fußnoten | yes |
| Fragmente | 3 |
Fragmente der Quelle:
| [1.] Arc/Fragment 044 15 - Diskussion Zuletzt bearbeitet: 2014-02-26 10:54:52 Hindemith | Arc, Fragment, Gesichtet, Rocic et al 2007, SMWFragment, Schutzlevel sysop, Verschleierung |
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| Untersuchte Arbeit: Seite: 44, Zeilen: 15-25 |
Quelle: Rocic et al 2007 Seite(n): H2730, Zeilen: left col. 33-47 |
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| For the in vitro studies in freshly isolated cardiomyocytes (Cardiomyocyte Isolation for details), G-CSF was administered for 10 min (total treatment time 30 min), and DHE was added during the last 20 min of treatment with G-CSF. Cells were then immediately observed under a fluorescent microscope. For in vivo studies, DHE was injected into the LV (60 μg/kg, n=3/group) for 20 min before two consecutive periods of ischemia-reperfusion (40s occlusion followed by 20min reperfusion and another 40s occlusion and 20min reperfusion). Animals were then sacrificed. The heart was removed, frozen in optimum cutting temperature compound on dry ice, and stored at -80°C until sectioning. Sections (5 μm) were made in a cryomicrotome and were mounted on glass slides. DHE fluorescence was detected with excitation/emission at 518/605 nm. All images were analyzed at the same microscope settings, and fluorescence intensity was obtained by Metamorph Software on three hearts (10 sections per heart). | For HCAEC cell culture, DHE was administered during the last 20 min of treatment. Cells were then observed immediately under a fluorescent microscope. For in vivo studies, DHE or DCF were injected into the LV (60 μg/kg) for 20 min before two consecutive periods of ischemia-reperfusion (40 s occlusion followed by 20 min reperfusion and another 40 s occlusion and 20 min reperfusion). Animals were then killed. The heart was removed, frozen in optimum cutting temperature compound on dry ice, and stored at -70°C until sectioning. Sections (5 μm) were made in a cryomicrotome and were mounted on glass slides. DHE or DCF fluorescence was detected with excitation/emission at 518/605 nm (for DHE) or 480/515 nm (for DCF). All images were analyzed at the same microscope settings, and relative fluorescence readings were obtained by Metamorph Software on three hearts (5 consecutive sections per heart). |
Though taken verbatim nothing has been marked as a citation and the source is not given. |
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| [2.] Arc/Fragment 046 25 - Diskussion Zuletzt bearbeitet: 2014-02-26 20:25:17 Hindemith | Arc, Fragment, Gesichtet, Rocic et al 2007, SMWFragment, Schutzlevel sysop, Verschleierung |
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| Untersuchte Arbeit: Seite: 46, Zeilen: 25-27 |
Quelle: Rocic et al 2007 Seite(n): H2730, Zeilen: right col. 3-8 |
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| The extent of tube formation was quantified after 24h by superimposing a grid (25mm2/cube) on microscopic images, and the number of squares containing tubes were counted and [averaged from five randomly selected fields for each well to obtain the percentage of total field that contained tubes.] | The extent of tube formation was quantified after two days by using Scion Image software. An electronic grid was superimposed on microscopic images, and the number of squares containing tubes were counted and averaged from five randomly selected fields for each well to obtain the percentage of total field that contained tubes. |
Nearly identical, but not marked as a citation. |
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| [3.] Arc/Fragment 047 01 - Diskussion Zuletzt bearbeitet: 2014-02-26 22:57:53 Schumann | Arc, Fragment, Gesichtet, Rocic et al 2007, SMWFragment, Schutzlevel sysop, Verschleierung |
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| Untersuchte Arbeit: Seite: 47, Zeilen: 1-5 (complete) |
Quelle: Rocic et al 2007 Seite(n): H2730, Zeilen: right col. 3-8, 22-26 |
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| [The extent of tube formation was quantified after 24h by superimposing a grid (25mm2/cube) on microscopic images, and the number of squares containing tubes were counted and] averaged from five randomly selected fields for each well to obtain the percentage of total field that contained tubes. Treatments were conducted in n=4.
3.9 Data analysis ANOVA followed by t-tests was used for statistical analysis. A probability value of P<0.05 was used to determine statistical significance. |
The extent of tube formation was quantified after two days by using Scion Image software. An electronic grid was superimposed on microscopic images, and the number of squares containing tubes were counted and averaged from five randomly selected fields for each well to obtain the percentage of total field that contained tubes.
[...] All experiments were performed in triplicate (n=3 animals/group). Data analysis. ANOVA followed by t-tests with Bonferroni inequalities was used for statistical analysis. A probability value of P<0.05 was used to determine statistical significance. |
Nearly identical, but not marked as a citation. |
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