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Angaben zur Quelle [Bearbeiten]

Autor     Sheeja Navakkode Gangadharan
Titel    Investigation of Cellular Mechanisms of Hippocampal LTP and LTD
Jahr    2006
Umfang    121 S.
Anmerkung    Magdeburg, Univ., Diss. (rer. nat.)
URL    http://diglib.uni-magdeburg.de/Dissertationen/2006/shenavakkode.pdf

Literaturverz.   

nein
Fußnoten    nein
Fragmente    2


Fragmente der Quelle:
[1.] Br/Fragment 038 07 - Diskussion
Zuletzt bearbeitet: 2016-05-21 18:22:17 Schumann
Br, Fragment, Gangadharan 2006, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

Typus
Verschleierung
Bearbeiter
Graf Isolan
Gesichtet
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Untersuchte Arbeit:
Seite: 38, Zeilen: 7-24
Quelle: Gangadharan 2006
Seite(n): 42, Zeilen: 6-21
Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and the dura was removed before transferring the brain into cooled and carbogenated (carbogen: gas consisting of 95% O2 and 5% CO2) artificial cerebrospinal fluid (ACSF) (temperature 40C) (Reymann et al., 1985). Cold solution was used to slow down the metabolism of the tissue, to limit the extent of excitotoxic and other kinds of damage occurring during the preparation of slices (Reymann et al., 1985). Cooling the petridish and tissue slicer support on ice may help to reduce tissue deterioration. Brain is placed in a petridish on filter paper and the cerebellum and frontal cortex is dissected away. Then the remaining part of the brain is divided in the central sulcus by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of a manuel chopper (Cambden, UK). The hippocampus was chopped into 400μm thick slices at 700 angle transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush floated in a glass vessel containing the cooled and carbogenated ACSF, and immediately transferred to the nylon net in the experimental chamber maintained at 320C by a wide mouthed pipette.

• Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15:249-255.

Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and dura removed before transferring the brain into chilled and carbogenated (carbogen: gas consisting of 95% O2 and 5% CO2) artificial cerebrospinal fluid (ACSF) (about 4°C) (Reymann et al., 1985). Cold solution was used to slow down the metabolism of the tissue, to limit the extent of excitotoxic and other kinds of damage occurring during the preparation of slices (Reymann et al., 1985). Chilling the petridish and tissue slicer support on ice may help reduce tissue deterioration. Brain is placed in a petriplate on filter paper and the cerebellum and frontal cortex is dissected away. Divide the remaining part of the brain in the central sulcus by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of manuel tissue chopper (Cambden, UK), and 400 μm thick slices were cut at 70° transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush, floated in a petri dish containing the cooled and carbogenated ACSF, and immediately transferred to the nylon net in the experimental chamber maintained at 32oC by a wide bored pipette.

133. Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15: 249-255.

Anmerkungen

Nothing has been marked as a citation.

Sichter
(Graf Isolan) Schumann

[2.] Br/Fragment 075 21 - Diskussion
Zuletzt bearbeitet: 2016-05-04 21:37:56 WiseWoman
Br, Fragment, Gangadharan 2006, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

Typus
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Bearbeiter
Graf Isolan
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Untersuchte Arbeit:
Seite: 75, Zeilen: 21-26
Quelle: Gangadharan 2006
Seite(n): 37, 90, Zeilen: 37:19-23; 90:5-8.(9-10)
The CaMKII holoenzymes were shown to be capable of associating with one another in response to Ca2+ [sic].Therefore CaMKII may form a scaffold that, in combination with other synaptic proteins, recruits and localizes additional proteins to the postsynaptic density (Hudmon et al., 2005). The atypical protein kinase C known as protein kinase M Zeta (PKMζ), the persistent activity of which requires protein synthesis, has been shown to be another possible candidate. But recently it has [been found that PKMζ is the first LTP specific plasticity-related protein (PRP) and not a tag molecule in apical CA1 branches (Sajikumar et al., 2005) but in basal dendrites the coactivation of PKA or PKM Zeta is required for synaptic tagging (Sajikumar et al., 2007).]

• Hudmon A, Lebel E, Roy H, Sik A, Schulman H, Waxham MN, De Koninck P (2005) A mechanism for Ca2+/calmodulin-dependent protein kinase II clustering at synaptic and nonsynaptic sites based on self-association. J Neurosci 25:6971-83.

• Sajikumar S, Navakkode S, Frey JU (2005) Protein synthesis-dependent long-term functional plasticity: Methods and techniques. Curr Opin Neurobiol 15:607-613.

• Sajikumar S, Navakkode S, Sacktor TC, Frey JU (2005b) Synaptic tagging and cross tagging: the role of protein kinase M zeta in maintaining long-term potentiation but not long-term depression. J Neurosci 25:5750-5756.

[page 37]

The atypical protein kinase C known as protein kinase Mζ (PKMζ), the persistent activity of which requires protein synthesis, has been shown to be another possible candidate. But recently we could identify PKMæ as the first LTP specific plasticity related protein, not a tag molecule (Sajikumar et al., 2005b).

[page 90]

More recently Hudmon et al showed that CaMKII holoenzymes were shown to be capable of associating with one another (or self-associate) in response to Ca2+ stimulation. Therefore CaMKII may form a scaffold that, in combination with other synaptic proteins, recruits and localizes additional proteins to the postsynaptic density. They also discussed the potential function of CaMKII self-association as a ´tag´ of synaptic activity (Hudmon et al., 2005). [...]


74. Hudmon A, Lebel E, Roy H, Sik A, Schulman H, Waxham MN, De Koninck P (2005) A mechanism for Ca2+/calmodulin-dependent protein kinase II clustering at synaptic and nonsynaptic sites based on self-association. J Neurosci 25: 6971-6983.

139. Sajikumar S, Navakkode S, Frey JU (2005a) Protein synthesis-dependent long-term functional plasticity: methods and techniques. Curr Opin Neurobiol ..

140. Sajikumar S, Navakkode S, Sacktor TC, Frey JU (2005b) Synaptic tagging and cross-tagging: the role of protein kinase Mzeta in maintaining long-term potentiation but not long-term depression. J Neurosci 25: 5750-5756.

Anmerkungen

From the final part - "Discussion" - of the thesis, Not marked as a citation.

Sichter
(Graf Isolan) Schumann

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