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Angaben zur Quelle [Bearbeiten]

Autor     M. Berger, Erwin-Josef Speckmann, Hans-Christian Pape, Ali Gorji
Titel    Spreading depression enhances human neocortical excitability in vitro
Zeitschrift    Cephalalgia
Datum    May 2008
Jahrgang    28
Nummer    5
Seiten    558-562
DOI    doi:10.1111/j.1468-2982.2008.01556.x
URL    http://cep.sagepub.com/content/28/5/558.full

Literaturverz.   

yes
Fußnoten    yes
Fragmente    4


Fragmente der Quelle:
[1.] Clg/Fragment 016 20 - Diskussion
Zuletzt bearbeitet: 2014-05-11 19:09:17 Schumann
Berger et al 2008, Clg, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

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Quelle: Berger et al 2008
Seite(n): 560, Zeilen: left col. 23-31, 45-50
Cortical SD-like events were evaluated with respect to their amplitude, duration and velocity rates. SD duration was defined as the interval between the time of half-maximal voltage shift during onset and recovery of the negative DC potential deflection.

3. Long-term potentiation

Single pulses of electrical stimulation were applied through a bipolar platinum electrode attached to the white matter perpendicular to the recording electrodes. Evoked field excitatory postsynaptic potentials (fEPSP) were recorded in the third layer of neocortical slices. The fEPSP was elicited by adjusting the intensity of stimulation to 50% of that at which population spikes after fEPSP began [to appear.]

Single pulses of electrical stimulation were

applied through a bipolar platinum electrode attached to the white matter perpendicular to the recording electrodes. Evoked field excitatory postsynaptic potentials (fEPSP) were recorded in the third layer of neocortical slices. The fEPSP was elicited by adjusting the intensity of stimulation to ~50% of that at which population spikes after fEPSP began to appear. [...] Spreading depression (SD)-like events were evaluated with respect to their repetition, amplitude, duration and velocity rates. SD duration was defined as the interval between the time of half-maximal voltage shift during onset and recovery of the negative d.c. potential deflection.

Anmerkungen

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Sichter
(Graf Isolan) Agrippina1

[2.] Clg/Fragment 017 01 - Diskussion
Zuletzt bearbeitet: 2014-05-11 19:06:27 Schumann
Berger et al 2008, Clg, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

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Quelle: Berger et al 2008
Seite(n): 560, Zeilen: left col. 31-45
The amplitude of fEPSP 1 ms after the onset was measured for data analysis. In long-term potentiation (LTP) experiments, the cortex was sequentially stimulated once every minute. Ten trains of four pulses (pulse duration 0.1 msec; interpulse interval 50 msec; intensity 5 V) were repeated at intervals of 10 msec. LTP was operationally defined as the mean change in fEPSP amplitude in response to five stimuli given 30 min after tetanic stimulation compared with the mean response to five test pulses applied immediately before the stimulation. Thus % potentiation = [(posttetanus amplitude of fEPSP/baseline amplitude of fEPSP) 1] 100. Tetanic stimulation was applied 60 min after application of drug. The amplitude of fEPSP 1 ms after the onset was measured for data analysis. In long-term potentiation (LTP) experiments, the cortex was sequentially stimulated once every minute. Ten trains of four pulses (pulse duration 0.1 ms; interpulse interval 50 ms; intensity 5 V) were repeated at intervals of 10 s. LTP was operationally defined as the mean change in fEPSP amplitude in response to five stimuli given 30 min after tetanic stimulation compared with the mean response to five test pulses applied immediately before the stimulation. Thus, percentage potentiation = post-tetanus amplitude of fEPSP/baseline amplitude of fEPSP. Tetanic stimulation was applied 30 min after induction of CSD.
Anmerkungen

The source is not given.

Sichter
(Graf Isolan) Agrippina1

[3.] Clg/Fragment 018 19 - Diskussion
Zuletzt bearbeitet: 2014-05-11 19:27:18 Schumann
Berger et al 2008, Clg, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

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Quelle: Berger et al 2008
Seite(n): 560, Zeilen: right col. 22-32
A conditioning tetanic stimulation was delivered to the white substance of neocortical slices followed by pulses with stimulation parameters identical to control values. The evoked fEPSP was stable for at least 30 min before application of tetanic stimulation (less than 10% variation; Fig. 5). Administration of tetanic stimulation produced a rapid and stable enhancement of the amplitude of the fEPSP in all tested preparations (n = 6, 153 ± 10 % control; Fig. 6). A conditioning tetanic stimulation was delivered to the white substance of neocortical slices after induction of CSD or injection of ACSF followed by pulses with stimulation parameters identical to control values. Evoked field potentials were stable for at least 30 min before application of tetanic stimulation (< 10% variation). Tetanic stimulation in slices treated by ACSF injection (control group) produced a rapid, stable and long-lasting enhancement of the amplitude of fEPSP in all tested preparations (n = 6, 130 ± 2.7% control).
Anmerkungen

Not marked as a citation.

Sichter
(Graf Isolan) Agrippina1

[4.] Clg/Fragment 021 02 - Diskussion
Zuletzt bearbeitet: 2014-05-11 22:54:14 Schumann
Berger et al 2008, Clg, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung

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Seite: 21, Zeilen: 2-7
Quelle: Berger et al 2008
Seite(n): 561, Zeilen: Figure 1: 12-20
Fig. 5: [...] (A) Tetanic stimulation

(Ten trains of four pulses; pulse duration 0.1 msec; interpulse interval 50 msec; intensity 5 V) produces a rapid and stable potentiation in the amplitude of the evoked field potentials, calculated as a percentage of baseline mean response amplitude. Open triangles, open square, and closed circles show the evoked fEPSP after application of somatostatin (500 nmol/l), and control, respectively. Arrow shows the time of tetanic stimulation, 60 min after [application of substances.]

Figure 1 [...] (B2) Tetanic stimulation (10 trains of four pulses, pulse duration 0.1 ms; interpulse interval 50 ms) produces a rapid and stable potentiation in the amplitude of fEPSP, calculated as a percentage of baseline mean response amplitude. Solid hexagons and open circles show the evoked fEPSP after induction of CSD and control, respectively. Arrow shows the time of tetanic stimulation, 45 min after KCl application and ACSF (control).
Anmerkungen

Nothing is marked as a citation.

Sichter
(Graf Isolan) Schumann

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