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Titel    Hematopoietic stem cell
Verlag    (Wikipedia)
Datum    30. December 2008
URL    http://en.wikipedia.org/w/index.php?title=Hematopoietic_stem_cell&oldid=260921212

Literaturverz.   

no
Fußnoten    no
Fragmente    4


Fragmente der Quelle:
[1.] Pak/Fragment 009 25 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:28:32 Hindemith
Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia hematopoietic stem cell 2008

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Quelle: Wikipedia hematopoietic stem cell 2008
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The CFU that can reside in the spleen and form a colony are called CFU-S. The colony-forming unit–spleen (CFU–S) depends on the ability of infused bone marrow cells to give rise to clones of maturing haematopoietic cells in the spleens of irradiated mice after 8 to 12 days. It is used to measure more mature progenitor or Transit Amplifying Cells rather than stem cells.26

26.Coulombel L. Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assays. Oncogene. 2004;23:7210-7222.

Another CFU, the colony-forming unit–spleen (CFU–S) was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. It was used extensively in early studies, but is now considered to measure more mature progenitor or Transit Amplifying Cells rather than stem cells.
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[2.] Pak/Fragment 011 07 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:28:28 Hindemith
Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia hematopoietic stem cell 2008

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The colony-forming unit–spleen (CFU–S) was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing haematopoietic cells in the spleens of irradiated mice after 8 to 12 days. It is considered to be a measure of more mature progenitor or Transit Amplifying Cells rather than stem cells. Another CFU, the colony-forming unit–spleen (CFU–S) was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. It was used extensively in early studies, but is now considered to measure more mature progenitor or Transit Amplifying Cells rather than stem cells.
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[3.] Pak/Fragment 016 06 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:34:00 Hindemith
Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia hematopoietic stem cell 2008

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Quelle: Wikipedia hematopoietic stem cell 2008
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Using limiting dilution strategies combined with other streamlined experimental and statistical methods for examining HSCs at the clonal level, HSCs fall into three distinct lineage-biased clusters.56-58 These are quantitatively defined by the ratio “ρ” of lymphoid to myeloid cells that the HSC generates upon differentiation (which makes ñ [sic] a peripheral predictor for the clonal association of a reconstituted haematopoietic system). Balanced HSCs repopulate peripheral white blood cells in the same ratio of myeloid to lymphoid cells as seen in unmanipulated mice (on average about 15% myeloid and 85% lymphoid cells, or 3≤ñ≤10 [sic]), in the NOD/SCID mice. Myeloid-biased (My-bi) HSC give rise to too few lymphocytes resulting in ratios 0<ρ<3, whereas lymphoid-biased (Ly-bi) HSC generate too few myeloid cells, which results in lymphoid-to-myeloid ratios of 10<ρ<00 [sic]. All three types are normal HSCs in the sense that they have self-renewal capacity and can regenerate all haematopoietic lineages (pluripotency).

56. Muller-Sieburg [sic] CE, Cho RH, Thoman M, Adkins B, Sieburg HB. Deterministic regulation of hematopoietic stem cell self-renewal and differentiation. Blood. 2002;100:1302-1309.

57. Muller-Sieburg [sic] CE, Cho RH, Karlsson L, Huang JF, Sieburg HB. Myeloid-biased hematopoietic stem cells have extensive self-renewal capacity but generate diminished lymphoid progeny with impaired IL-7 responsiveness. Blood. 2004;103:4111-4118.

58. Sieburg HB, Cho RH, Dykstra B, Uchida N, Eaves CJ, Muller-Sieburg [sic] CE. The hematopoietic stem compartment consists of a limited number of discrete stem cell subsets. Blood. 2006;107:2311-2316.

Using limiting dilution strategies combined with other streamlined experimental and statistical methods for examining HSCs at the clonal level, it was shown that HSCs fall into three distinct lineage-bias[1] [2] [3] clusters. These are quantitatively defined by the ratio ρ of lymphoid to myeloid cells that HSC generate upon differentiation (which makes ρ a peripheral predictor for the clonal association of a reconstituted hematopoietic system). Balanced HSCs repopulate peripheral white blood cells in the same ratio of myeloid to lymphoid cells as seen in unmanipulated mice (on average about 15% myeloid and 85% lymphoid cells, or 3≤ρ≤10). Myeloid-biased (My-bi) HSC give rise to too few lymphocytes resulting in ratios 0<ρ<3, whereas lymphoid-biased (Ly-bi) HSC generate too few myeloid cells, which results in lymphoid-to-myeloid ratios of 10 < ρ < oo. All three types are normal HSC in that they have self-renewal capacity and can regenerate all hematopietic [sic] lineages (pluripotency).

1. Muller-Sieburg [sic] CE, Cho RH, Thoman M, Adkins B, Sieburg HB, Deterministc [sic] regulation of hematopoietic stem cell self-renewal and differentiation. Blood. 2002; 100; 1302-9

2. Muller-Sieburg [sic] CE, Cho RH, Karlson [sic] L, Huang JF, Sieburg HB. Myeloid-biased hematopoietic stem cells have extensive self-renewal capacity but generate diminished progeny with impaired IL-7 responsiveness. Blood. 2004; 103:4111-8.

3. Sieburg HB, Cho RH, Dykstra B, [sic] Eaves, CJ, Muller-Sieburg [sic], CE. The hematopoietic stem cell [sic] compartment consists of a limited number of discrete stem cell subsets. Blood. 2006; 107:2311-6. Epub 2005 Nov 15.

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[4.] Pak/Fragment 085 02 - Diskussion
Zuletzt bearbeitet: 2014-04-06 08:36:27 Hindemith
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Balanced HSCs repopulate peripheral white blood cells in the same ratio of myeloid to lymphoid cells as seen in unmanipulated mice (on average about 15% myeloid and 85% lymphoid cells, or 3≤ñ≤10 [sic]). Balanced HSCs repopulate peripheral white blood cells in the same ratio of myeloid to lymphoid cells as seen in unmanipulated mice (on average about 15% myeloid and 85% lymphoid cells, or 3≤ρ≤10).
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