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Quelle:Sih/Giaever und Keese 1983

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Angaben zur Quelle [Bearbeiten]

Autor     Ivar Giaever, Charles R. Keese
Titel    Behavior of cells at fluid interfaces (protein adsorption at interfaces/fluorocarbon fluid substrates/cellular growth patterns)
Zeitschrift    Proceedings of the National Academy of Sciences of the United States of America
Ausgabe    80
Datum    Januar 1983
Nummer    1
Seiten    219-222
Anmerkung    Pubmed PMC393343
URL    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC393343/pdf/pnas00627-0234.pdf

Literaturverz.   

ja
Fußnoten    nein
Fragmente    6


Fragmente der Quelle:
[1.] Analyse:Sih/Fragment 041 01 - Diskussion
Zuletzt bearbeitet: 2014-08-28 21:16:36 Graf Isolan
Fragment, Giaever und Keese 1983, KomplettPlagiat, SMWFragment, Schutzlevel, Sih, ZuSichten

Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
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Untersuchte Arbeit:
Seite: 41, Zeilen: 1-10
Quelle: Giaever und Keese 1983
Seite(n): 219, Zeilen: li. Sp. 22ff
In 1964 Rosenberg introduced the use of a fluid substrate for the growth of both transformed and anchorage-dependent cells 284, 285. In this method, a cell suspension is introduced over an inert hydrophobic liquid having a density greater than that of the aqueous medium, and cells are observed to spread and divide on the liquid│liquid interface between the two immiscible phases. As is the case for solid substrates, the cells do not interact directly with the interface but rather with proteins that adsorb to the interfacial junction. These proteins presumably denature as their polypeptide chains unfold to achieve a low energy orientation with most of the hydrophilic portions exposed to the aqueous phase and the hydrophobic portions in the inert nonaqueous phase.

284. Rosenberg MD (1964) In Cellular Control Mechanisms and Cancer. Elsevier, Amsterdam

285. Rosenberg MD (1965) In Baroda Tissue Culture Seminar. Junk, The Hague, The Netherlands

In 1964 Rosenberg introduced the use of a fluid substrate for the growth of both transformed and anchorage-dependent cells (2, 3). In this method a cell suspension is introduced over an inert hydrophobic liquid having a density greater than that of the aqueous medium, and cells are observed to spread and divide on the liquid-liquid interface between the two immiscible phases. As is the case for solid substrates, the cells do not interact directly with the interface but rather with proteins that adsorb to the interfacial junction. These proteins presumably denature as their polypeptide chains unfold to achieve a low energy orientation with most of the hydrophillic portions exposed to the aqueous phase and the hydrophobic portions in the inert nonaqueous phase (4).

2. Rosenberg, M. D. (1964) in Cellular Control Mechanisms and Cancer, eds. Emmelot, P. & Mihlbock, 0. (Elsevier, Amsterdam), pp. 146-164.

3. Rosenberg, M. D. (1965) in Baroda Tissue Culture Seminar, ed. Ramakrishnan, C. V. (Junk, The Hague, The Netherlands), pp. 93-107.

4. Shaw, D. J. (1966) Introduction to Collid and Surface Chemistry (Butterworths, London), p. 57.

Anmerkungen

Ohne Hinweis auf eine Übernahme. Die Passage wird schon einmal später in der Dissertation (ohne Nachweis) verwendet (vgl. Sih/Fragment_175_03).

Sichter
(Graf Isolan)

[2.] Analyse:Sih/Fragment 042 01 - Diskussion
Zuletzt bearbeitet: 2014-08-31 21:02:31 Graf Isolan
Fragment, Giaever und Keese 1983, SMWFragment, Schutzlevel, Sih, Verschleierung, ZuSichten

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Untersuchte Arbeit:
Seite: 42, Zeilen: 1-4, 5-10
Quelle: Giaever und Keese 1983
Seite(n): 219, 221, Zeilen: 219:li.Sp. 46-49 - re.Sp. 1-3; 221:re.Sp. 4-7
Cells grown on such substrates can be transferred by simply pipetting the cell layer. Such a procedure of cell harvesting is especially important where the effect of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells should be avoided. The compositions of cell membrane proteins are changed by enzymatic treatment or by mechanical scraping 301. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments. If the adsorbed serum proteins at the interface are crosslinked by glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat 88, the patterns of cell growth can be altered significantly.

88. Feder J, Giaever I (1980) Adsorption of ferritin. J Colloid Interface Sci 78 144-54

301. Shaw DJ (1966) Introduction to Collid and Surface Chemistry. Butterworths, London

[Seite 219]

In addition, cells grown on such substrates can be transferred from one fluid interface to another or from a fluid to a solid substrate by simply pipetting the cell layer. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments.

[Seite 221]

If the adsorbed serum proteins at the interface. are crosslinked by using glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat (6), the patterns of cell growth can be altered significantly.


4. Shaw, D. J. (1966) Introduction to Collid and Surface Chemistry (Butterworths, London), p. 57.

6. Feder, J. & Giaever, I. (1980) J. Colloid Interface Sci. 78, 144-154.

Anmerkungen

Ohne Hinweis auf eine Übernahme. Formulierungen der Vorlage werden an dieser Stelle gleich zweimal verwendet. Auf Seite 175 wiederholt sich das Spiel (vgl. Sih/Fragment_175_17).

Sichter
(Graf Isolan)

[3.] Analyse:Sih/Fragment 042 18 - Diskussion
Zuletzt bearbeitet: 2014-08-28 21:31:11 Graf Isolan
Fragment, Giaever und Keese 1983, KomplettPlagiat, SMWFragment, Schutzlevel, Sih, ZuSichten

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Untersuchte Arbeit:
Seite: 42, Zeilen: 18-21
Quelle: Giaever und Keese 1983
Seite(n): 219, Zeilen: li.Sp. 41-46
The fluorocarbon│aqueous interface is well suited to provide an inert, nontoxic, hydrophobic substrate for cell growth. It has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces, which, in general, have polar molecular inhomogeneities. We have found the fluorocarbon interface to be well suited to provide an inert, nontoxic, hydrophobic substrate for cell growth. It has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces which, in general, have polar molecular inhomogeneities (3, 5).

3. Rosenberg, M. D. (1965) in Baroda Tissue Culture Seminar, ed. Ramakrishnan, C. V. (Junk, The Hague, The Netherlands), pp. 93-107.

5. Maroudas, N. G. (1973) Nature (London) 244, 353-354.

Anmerkungen

Ohne Hinweis auf eine Übernahme.

Sichter
(Graf Isolan)

[4.] Analyse:Sih/Fragment 175 03 - Diskussion
Zuletzt bearbeitet: 2014-08-28 21:15:35 Graf Isolan
Fragment, Giaever und Keese 1983, KomplettPlagiat, SMWFragment, Schutzlevel, Sih, ZuSichten

Typus
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Graf Isolan
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Untersuchte Arbeit:
Seite: 175, Zeilen: 3-9
Quelle: Giaever und Keese 1983
Seite(n): 219, Zeilen: li. Sp. 22ff
In 1964 Rosenberg introduced the use of a fluid substrate for the growth of both transformed and anchorage-dependent cells 284, 285. In this method a cell suspension is introduced over an inert hydrophobic liquid having a density greater than that of the aqueous medium, and cells are observed to spread and divide at the liquid│liquid interface between the two immiscible phases. As is the case for solid substrates, the cells do not interact directly with the interface but rather with proteins that adsorb to the interfacial junction.

284. Rosenberg MD (1964) In Cellular Control Mechanisms and Cancer. Elsevier, Amsterdam

285. Rosenberg MD (1965) In Baroda Tissue Culture Seminar. Junk, The Hague, The Netherlands

In 1964 Rosenberg introduced the use of a fluid substrate for the growth of both transformed and anchorage-dependent cells (2, 3). In this method a cell suspension is introduced over an inert hydrophobic liquid having a density greater than that of the aqueous medium, and cells are observed to spread and divide on the liquid-liquid interface between the two immiscible phases. As is the case for solid substrates, the cells do not interact directly with the interface but rather with proteins that adsorb to the interfacial junction.

2. Rosenberg, M. D. (1964) in Cellular Control Mechanisms and Cancer, eds. Emmelot, P. & Mihlbock, 0. (Elsevier, Amsterdam), pp. 146-164.

3. Rosenberg, M. D. (1965) in Baroda Tissue Culture Seminar, ed. Ramakrishnan, C. V. (Junk, The Hague, The Netherlands), pp. 93-107.

Anmerkungen

Ohne Hinweis auf eine Übernahme. Die Passage wurde schon einmal in der Dissertation (ohne Nachweis) verwendet (vgl. Sih/Fragment_041_01).

Sichter
(Graf Isolan)

[5.] Analyse:Sih/Fragment 175 17 - Diskussion
Zuletzt bearbeitet: 2014-08-31 21:07:53 Graf Isolan
Fragment, Giaever und Keese 1983, SMWFragment, Schutzlevel, Sih, Verschleierung, ZuSichten

Typus
Verschleierung
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Graf Isolan
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Untersuchte Arbeit:
Seite: 175, Zeilen: 17-22, 24-29
Quelle: Giaever und Keese 1983
Seite(n): 219, 221, Zeilen: 219:li.Sp. 43-49 - re.Sp. 1-3; 221:re.Sp. 4-7
This method of cell culture has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces, which, in general, have polar molecular inhomogeneities. In addition, cells grown on such substrates can be transferred by simply pipetting the cell layer. Such procedure of cell harvesting is especially important where the effect of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells can be avoided. It is here to be mentioned that the compositions of cell membrane proteins are changed by enzymatic treatment or by mechanical scraping 301. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments. If the adsorbed serum proteins at the interface are crosslinked by glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat 88, the patterns of cell growth can be altered significantly.

88. Feder J, Giaever I (1980) Adsorption of ferritin. J Colloid Interface Sci 78 144-54

301. Shaw DJ (1966) Introduction to Collid and Surface Chemistry. Butterworths, London

[Seite 219]

It has the advantage of being exceptionally homogeneous and reproducible when compared with hydrophobic solid surfaces which, in general, have polar molecular inhomogeneities (3, 5). In addition, cells grown on such substrates can be transferred from one fluid interface to another or from a fluid to a solid substrate by simply pipetting the cell layer. Such a procedure is of particular interest in that it obviates the use of trypsin or other proteolytic enzymes or chelating agents to passage cultured cells and avoids the uncertain effects of such treatments.

[Seite 221]

If the adsorbed serum proteins at the interface. are crosslinked by using glutaraldehyde or if a bimolecular layer of proteins is formed by using polylysine as a base coat (6), the patterns of cell growth can be altered significantly.


3. Rosenberg, M. D. (1965) in Baroda Tissue Culture Seminar, ed. Ramakrishnan, C. V. (Junk, The Hague, The Netherlands), pp. 93-107.

4. Shaw, D. J. (1966) Introduction to Collid and Surface Chemistry (Butterworths, London), p. 57.

5. Maroudas, N. G. (1973) Nature (London) 244, 353-354.

6. Feder, J. & Giaever, I. (1980) J. Colloid Interface Sci. 78, 144-154.

Anmerkungen

Ohne Hinweis auf eine Übernahme. Die Formulierungen der Vorlage werden an dieser Stelle gleich zweimal verwendet. Dasselbe ist schon mal auf Seite 42 passiert (vgl. Sih/Fragment_042_01).

Sichter
(Graf Isolan)

[6.] Analyse:Sih/Fragment 176 01 - Diskussion
Zuletzt bearbeitet: 2014-09-01 16:41:52 Graf Isolan
Fragment, Giaever und Keese 1983, SMWFragment, Schutzlevel, Sih, Verschleierung, ZuSichten

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Untersuchte Arbeit:
Seite: 176, Zeilen: 1-3
Quelle: Giaever und Keese 1983
Seite(n): 219, Zeilen: li.Sp. 38-41
It is a matter of regret that since the original work was published in 1964, there seems to have been relatively little interest in the growth of cells on fluid substrates in spite of many advantages not afforded by solid substrates in culture. Since the original work was published, there seems to have been relatively little interest in the growth of cells on fluid substrates in spite of some advantages not afforded by solid substrates in studying cell behavior in culture.
Anmerkungen

Ohne Hinweis auf eine Übernahme.

Sichter
(Graf Isolan)

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