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Angaben zur Quelle [Bearbeiten]

Autor     YuanXiang Pan
Titel    Poly(A)+ RNA from sheep omasal epithelium induces expression of a peptide transport protein(s) in Xenopus laevis oocytes
Datum    August 1996
Anmerkung    Thesis submitted to the Graduate Faculty of the Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE in Animal Science
URL    http://scholar.lib.vt.edu/theses/public/etd-3856112379652351/etd.pdf

Literaturverz.   

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3.3 Xenopus laevis oocytes

One of the first and still most widely used assay system for quantifying an authentic protein biosynthetic process is the fully grown oocyte of the South African clawed frog, Xenopus laevis. The value of Xenopus laevis first became apparent in 1971, when Gurdon and co-workers discovered that the oocyte constitutes an efficient system for translating foreign messenger RNA.

The Xenopus oocyte is a cell specialized for the production and storage of proteins for later use during embryogenesis and developmentally divided into 6 stages (112). In addition, the complex architecture of the frog oocyte includes the subcellular systems involved in the export and import of proteins. Therefore, the mRNA-microinjected oocyte is an appropriate system to study the synthesis of specific polypeptides, as well as the storage of particular proteins in various subcellular organelles and the export of others into the extracellular space. Moreover, the subcellular compartmentalization, as well as the structure and biochemical, physiological, and biological properties of the synthesized protein, may be examined from exogenous proteins in the injected oocyte.


112. Costa,PF, Emilio,MG, Fernandes,PL, Ferreira,HG, Ferreira,KG: Determination of ionic permeability coefficients of the plasma membrane of Xenopus laevis oocytes under voltage clamp. J.Physiol 413:199-211, 1989

[Page 17]

Use of Xenopus Oocytes as a Model System. One of the first and still most widely used assay system for quantifying an authentic protein biosynthetic processes is the fully grown oocyte of the South African clawed frog, Xenopus laevis. The value of Xenopus laevis first became apparent in 1971 when Gurdon and co-workers discovered that the oocyte constitutes an efficient system for translating foreign messenger RNA (Gurdon et al., 1971).

The Xenopus oocyte is a cell specialized for the production and storage of proteins for later use during embryogenesis. In addition, the complex architecture of the

[Page 18]

frog oocyte includes the subcellular systems involved in the export and import of proteins. Therefore, the mRNA-microinjected oocyte is an appropriate system in which to study the synthesis of specific polypeptides, as well as the storage of particular proteins in various subcellular organelles and the export of others into the extracellular space. Moreover the subcellular compartmentalization, as well as the structure and biochemical, physiological, and biological properties of the synthesized protein, may be examined in the injected oocyte.


Gurdon, J. B., C. D. Lane, H. R. Woodland, and G. Marbaix. 1971. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature. 233: 177-182.

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[2.] Sj/Dublette/Fragment 025 04 - Diskussion
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In summary, the Xenopus oocyte system has the advantage that channels, receptors and transporters can rapidly be expressed and identified by their electrophysiological properties. Once cDNA clones have been isolated, oocytes are an excellent system for correlating structure with function using a combination of molecular biological and electrophysiological techniques and analyzed both biochemically and electro physiologically in an in vivo situation. In summary, the Xenopus oocyte system has the advantage that channels, receptors and transporters can be rapidly expressed and analyzed both biochemically and

electrophysiologically in an in vivo situation. The system can be used quite effectively as an assay for the functional cloning of channels that have only been identified by their electrophysiological properties. Once cDNA clones have been isolated, oocytes are an excellent system for correlating structure with function using a combination of molecular biological and electrophysiological techniques.

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