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Angaben zur Quelle [Bearbeiten]

Autor     Yi Fu, Jian Zhang, and Joseph R. Lakowicz
Titel    Metal-Enhanced Fluorescence of Single Green Fluorescent Protein (GFP)
Zeitschrift    Biochem Biophys Res Commun.
Datum    28. November 2008
Seiten    712–717
DOI    10.1016/j.bbrc.2008.09.062.
URL    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613175/

Literaturverz.   

yes
Fußnoten    yes
Fragmente    1


Fragmente der Quelle:
[1.] Tim/Fragment 023 19 - Diskussion
Zuletzt bearbeitet: 2014-10-27 04:13:04 Hindemith
BauernOpfer, Fragment, Fu 2008, Gesichtet, SMWFragment, Schutzlevel sysop, Tim

Typus
BauernOpfer
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 23, Zeilen: 19-26
Quelle: Fu 2008
Seite(n): abstract, p.6, Zeilen: p.6: last lines
In the study of 2008, Fu et al. observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking. The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection. In this report, we observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking.

The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection.

Anmerkungen

The source is given, but it is not clear to the reader how much has been taken from it. In particular the two sentences starting with "Accordingly, we observed" cannot be attributed to the source by the reader who rather assumes that the author herself made observations that are consistent with the findings of the source.

Sichter
(SleepyHollow02), Hindemith

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