Fandom

VroniPlag Wiki

Quelle:Tim/Schaefer 2007

< Quelle:Tim

31.354Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.

Angaben zur Quelle [Bearbeiten]

Autor     Lars V. Schäfer / Gerrit Groenhof / Astrid R. Klingen / G. Matthias Ullmann / Martial Boggio-Pasqua / Michael A. Robb / Helmut Grubmüller
Titel    Photoswitching of the Fluorescent Protein asFP595: Mechanism, Proton Pathways, and Absorption Spectra
Zeitschrift    Angew. Chem.
Jahr    2007
Seiten    536 –542
DOI    10.1002/ange.200602315

Literaturverz.   

yes
Fußnoten    yes
Fragmente    2


Fragmente der Quelle:
[1.] Tim/Fragment 015 11 - Diskussion
Zuletzt bearbeitet: 2014-10-27 05:38:51 Hindemith
BauernOpfer, Fragment, Gesichtet, SMWFragment, Schaefer 2007, Schutzlevel sysop, Tim

Typus
BauernOpfer
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 15, Zeilen: 11-15
Quelle: Schaefer 2007
Seite(n): 540, Zeilen: l.col.: 3 ff.
The proposed kindling mechanism (Schafer et al., 2007; Andresen et al., 2005) is depicted in figure 8. Before the photon absorption the chromophore is in the dark zwitterionic trans state but when the photon arrives it changes in the cis state. The isomerization increases the acidity of the imidazolinone NH proton of the zwitterionic cis chromophore and induces proton transfer to Glu215 (state A-cis, figure 8).

Whether this proton transfer takes place in the electronic ground state or in the excited state cannot be judged on the basis of the current simulations. The proton transfer also [prevents photoisomerization of the zwitterionic cis species back to the initial dark trans state.]

The proposed kindling mechanism is depicted in Figure 3. As shown previously, kindling is initiated by a trans–cis photoisomerization through a space-saving hula-twist mechanism.[ 11] The isomerization increases the acidity of the imidazolinone NH proton of the zwitterionic cis chromophore and induces proton transfer to Glu215 (state A-cis, Figure 3). Whether this proton transfer takes place in the electronic ground state or in the excited state cannot be judged on the basis of the current simulations. The proton transfer also prevents photoisomerization of the zwitterionic cis species back to the initial dark trans state.
Anmerkungen

The source is given together with another source such that a literal quote cannot be understood. This is particularly the case in the second paragraph, as it describes "current simulations".

Sichter
(SleepyHollow02), Hindemith

[2.] Tim/Fragment 016 01 - Diskussion
Zuletzt bearbeitet: 2014-10-27 05:46:23 Hindemith
BauernOpfer, Fragment, Gesichtet, SMWFragment, Schaefer 2007, Schutzlevel sysop, Tim

Typus
BauernOpfer
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 16, Zeilen: 1-7
Quelle: Schaefer 2007
Seite(n): 540, Zeilen: l.col.: 11 ff.
[The proton transfer also] prevents photoisomerization of the zwitterionic cis species back to the initial dark trans state. The anionic cis intermediate is subsequently protonated at the phenolate oxygen atom to finally yield the stable neutral chromophore (state N-cis).

This situation is similar to that in GFP, where the neutral protonation state is also favored for the cis chromophore (Helms, 2002; Stoner-Ma et al., 2005; Agmon, 2005; Leiderman et al., 2006; Vendrell et al., 2006). GFP fluorescence originates from the anionic chromophore, which is formed through excited-state proton transfer (ESPT).

The proton transfer also prevents photoisomerization of the zwitterionic cis species back to the initial dark trans state. The anionic cis intermediate is subsequently protonated at the phenolate oxygen atom to finally yield the stable neutral chromophore (state Ncis). This situation is similar to that in GFP, where the neutral protonation state is also favored for the cis chromophore.[16–20] GFP fluorescence originates from the anionic chromophore, which is formed through excited-state proton transfer (ESPT).

[16] V. Helms, Curr. Opin. Struct. Biol. 2002, 12, 169.

[17] D. Stoner-Ma, A. A. Jaye, P. Matousek, M. Towrie, S. R. Meech, P. J. Tonge, J. Am. Chem. Soc. 2005, 127, 2864.

[18] N. Agmon, Biophys. J. 2005, 88, 2452.

[19] P. Leiderman, D. Huppert, N. Agmon, Biophys. J. 2006, 90, 1009.

[20] O. Vendrell, R. Gelabert, M. Moreno, J. M. Lluch, J. Am. Chem. Soc. 2006, 128, 3564.

Anmerkungen

The source is given on the previous page.

Sichter
(SleepyHollow02), Hindemith

Auch bei Fandom

Zufälliges Wiki