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Angaben zur Quelle [Bearbeiten]

Autor     Aynur Tasdemir, Farid Khan, Thomas A. Jowitt, Lucia Iuzzolino, Stefan Lohmer, Sabrina Corazza, Thomas J. Schmidt
Titel    Engineering of a monomeric fluorescent protein AsGFP499 and its applications in a dual translocation and transcription assay
Zeitschrift    Protein Engineering, Design & Selection
Ausgabe    21
Jahr    2008
Nummer    10
Seiten    613-622
DOI    10.1093/protein/gzn040
URL    http://peds.oxfordjournals.org/content/21/10/613.full.pdf

Literaturverz.   

yes
Fußnoten    yes
Fragmente    1


Fragmente der Quelle:
[1.] Tim/Fragment 008 10 - Diskussion
Zuletzt bearbeitet: 2014-11-27 22:14:52 Hindemith
BauernOpfer, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Tasdemir et al 2008, Tim

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Untersuchte Arbeit:
Seite: 8, Zeilen: 10-19
Quelle: Tasdemir et al 2008
Seite(n): 613, 621, Zeilen: 613: r. col: 9 ff.; 621: r.col: 17 ff.
Contrary to monomeric AvGFP, all known Anthozoa GFP-like proteins reported to date form oligomers and exist mostly as tetramers (Baird et al., 2000; Mizuno et al., 2001; Wiedenmann et al., 2002; Shagin et al., 2004). The oligomerisation does not impair their application as reporter genes, selection markers or biosensors, but limits their use as fusion tags to study, for instance, protein localization and dynamics in living cells (Baird et al., 2000; Lauf et al., 2001; Mizuno et al., 2001). A possible solution to this problem is the genetic engineering of the fluorescent protein of interest by mutagenesis, creating mutants that form functional monomeric variants. For example, Tasdemir et al. (2008) converted the tetrameric AsGFP into dimeric and monomeric variants guided by a rational strategy of sequence alignments. Contrary to monomeric AvGFP, all known Anthozoa GFP-like proteins reported to date form oligomers and exist mostly as tetramers (Baird et al., 2000; Vrzheshch et al., 2000; Mizuno et al., 2001; Wiedenmann et al., 2002; Shagin et al., 2004). The oligomerisation does not impair their application as reporter genes, selection markers or biosensors, but limits their use as fusion tags to study, for instance, protein localisation and dynamics in living cells (Baird et al., 2000; Lauf et al., 2001; Mizuno et al., 2001). A possible solution to this problem is the genetic engineering of the fluorescent protein (FP) of interest by mutagenesis, creating mutants that form functional monomeric variants.

[page 621]

In summary, the present investigations led to a successful conversion of tetrameric AsGFP499 into dimeric and monomeric variants guided by a rational strategy of sequence alignments.

Anmerkungen

The source is mentioned, but as an illustration ("For example [...]") of the previously said and not as a reference of a source for it.

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(Hindemith), SleepyHollow02

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