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Autor     David E Root, Nir Hacohen, William C Hahn, Eric S Lander, David M Sabatini
Titel    Genome-scale loss-of-function screening with a lentiviral RNAi library
Zeitschrift    Nature methods
Ausgabe    3
Jahr    2006
Seiten    715 - 719
DOI    10.1038/nmeth924
URL    http://www.nature.com/nmeth/journal/v3/n9/full/nmeth924.html , Abbildung: [1]

Literaturverz.   

ja
Fußnoten    ja
Fragmente    1


Fragmente der Quelle:
[1.] Ww/Fragment 029 03 - Diskussion
Zuletzt bearbeitet: 2014-10-30 08:32:57 Hindemith
Fragment, Gesichtet, Root et al 2006, SMWFragment, Schutzlevel sysop, Verschleierung, Ww

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Verschleierung
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Hindemith
Gesichtet
Yes
Untersuchte Arbeit:
Seite: 29, Zeilen: 3-11
Quelle: Root et al 2006
Seite(n): 716, Zeilen: figure 1
Ww 29a diss

Fig. 2. Map for the pLKO.1 lentiviral vector. It derives from pRRLSIN.cPPT.PGK/GFP/WRPE and contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/5’LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5’LTR. SIN 3’LTR is the HIV 3’LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene.

Ww 29a source

Figure 1 Vector map for the pLKO.1 lentiviral vector. The self-inactivating lentiviral vector backbone contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/ 5′ LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5′ LTR. SIN 3′ LTR is the HIV 3′ LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene.

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