Quelle: Root et al 2006 Seite(n): 716, Zeilen: figure 1
Fig. 2. Map for the pLKO.1 lentiviral vector. It derives from pRRLSIN.cPPT.PGK/GFP/WRPE
and contains elements for efficient viral packaging and shRNA expression. These include Ψ,
the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/5’LTR is a hybrid
of the Rous Sarcoma virus promoter and the HIV 5’LTR. SIN 3’LTR is the HIV 3’LTR with a
self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter
(hU6). The lentiviral vector also contains the mammalian selection marker puromycin
resistance gene (PAC) under the control of the PGK promoter as a mammalian selection
marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded
phage F1 origin of replication, and AmpR is the ampicillin resistance gene.
Figure 1 Vector map for the pLKO.1 lentiviral vector. The self-inactivating
lentiviral vector backbone contains elements for efficient viral packaging
and shRNA expression. These include Ψ, the lentiviral packaging site;
RRE, the Rev-responsive element; cPPT. RSV/ 5′ LTR is a hybrid of the Rous
Sarcoma virus promoter and the HIV 5′ LTR. SIN 3′ LTR is the HIV 3′ LTR
with a self-inactivating U3 deletion. Expression of the shRNA is driven
by the human U6 promoter (hU6). The lentiviral vector also contains the
mammalian selection marker puromycin resistance gene (PAC) under the
control of the PGK promoter as a mammalian selection marker. pUCori is the
bacterial origin of replication of the plasmid, F1 ori is the single-stranded
phage F1 origin of replication, and AmpR is the ampicillin resistance gene.
Anmerkungen
Ein Verweis auf die Quelle fehlt hier. Sie wird auf der folgenden Seite zusammen mit zwei anderen Quellen genannt.