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Titel | Small hairpin RNA |
Verlag | (Wikipedia) |
Datum | 25. Juli 2007 |
URL | http://en.wikipedia.org/w/index.php?title=Small_hairpin_RNA&oldid=146938414 |
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Fußnoten | nein |
Fragmente | 2 |
[1.] Ww/Fragment 005 20 - Diskussion Zuletzt bearbeitet: 2016-02-03 22:18:56 Schumann | Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Wikipedia Small hairpin RNA 2007, Ww |
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Short hairpin RNAs (shRNAs) are a sequence of RNAs that makes a tight hairpin turn and are transcribed by RNA polymerase Ⅲ. It uses a vector introduced into cells and utilizes the U6 promoter to ensure that the shRNA is always expressed and can be cleaved by the cellular machinery into siRNA, which is then bound to the RISC silencing gene expression via RNAi (Brummelkamp et al., 2002; Lee et al., 2002; Miyagishi and Tiara, 2002; [Paddison, et al. 2002; Paul et al., 2002; Sui et al., 2002; Cao et al, 2005; Harper et al., 2005; McIntyre and Fanning, 2006).] | A short hairpin RNA (shRNA) is a sequence of RNA that makes a tight hairpin turn that can be used to silence gene expression via RNA interference. shRNA uses a vector introduced into cells and utilizes the U6 promoter to ensure that the shRNA is always expressed. This vector is usually passed on to daughter cells, allowing the gene silencing to be inherited. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex (RISC).
[...] References McIntyre G, Fanning G (2006). "Design and cloning strategies for constructing shRNA expression vectors". BMC Biotechnol. 6: 1. PMID 16396676. Harper S, Staber P, He X, Eliason S, Martins I, Mao Q, Yang L, Kotin R, Paulson H, Davidson B (2005). "RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model". Proc. Natl. Acad. Sci. U.S.A. 102 (16): 5820–5. PMID 15811941. Nielsen M, Pedersen F, Kjems J (2005). "Molecular strategies to inhibit HIV-1 replication". Retrovirology 2: 10. PMID 15715913. Paddison P, Caudy A, Bernstein E, Hannon G, Conklin D (2002). "Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells". Genes Dev. 16 (8): 948–58. PMID 11959843. Cao W, Hunter R, Strnatka D, McQueen C, Erickson R (2005). "DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response". J. Appl. Genet. 46 (2): 217–25. PMID 15876690. |
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[2.] Ww/Fragment 007 01 - Diskussion Zuletzt bearbeitet: 2014-10-29 17:07:10 Hindemith | Fragment, Gesichtet, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Wikipedia Small hairpin RNA 2007, Ww |
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shRNAs can also be made for use in plants and other systems, and are not necessarily driven by a U6 promoter. In plants the traditional promoter for strong constitutive [sic] expression (in most plant species) is the cauliflower mosaic virus 35S promoter (CaMV35S), in which case RNA polymerase II is used to express the transcript destined to initiate RNAi. | shRNAs can also be made for use in plants and other systems, and are not necessarily driven by a U6 promoter. In plants the traditional promoter for strong consitutive expression (in most plant species) is the cauliflower mosaic virus 35S promoter (CaMV35S), in which case RNA Polymerase II is used to express the transcript destined to initiate RNAi |
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