Rlm/043

Androgen Receptor and PIN1 in Prostate Cancer

von Dott. Raffaele La Montagna

vorherige Seite | zur Übersichtsseite | folgende Seite

F

Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende

[1.] Rlm/Fragment 043 09 - Diskussion Zuletzt bearbeitet: 2014-12-06 20:09:49 Singulus

BauernOpfer, Fragment, Gesichtet, Gordon et al 2010, Rlm, SMWFragment, Schutzlevel sysop

Typus BauernOpfer

Bearbeiter Graf Isolan

Gesichtet

Untersuchte Arbeit: Seite: 43, Zeilen: 9-17

Quelle: Gordon et al 2010 Seite(n): 2267, Zeilen: 21 ff.

Recently Gioeli et.al found an association between (CDK)9 and AR. CDK9 phosphorylates the AR on Ser81 in vitro. Phosphorylation is specific to AR Ser81 because CDK9 did not phosphorylate the AR on other serine phosphorylation sites. Overexpression of CDK9 with its cognate cyclin, Cyclin T, increased Ser81 phosphorylation levels in cells. Small interfering RNA knockdown of CDK9 protein levels decreased hormone-induced S81 phosphorylation. Additionally, treatment of LNCaP cells with the CDK9 inhibitors, 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole and Flavopiridol, reduced [Ser81 phosphorylation further, suggesting that CDK9 regulates Ser81 phosphorylation.]

We observed cyclin-dependent kinase (CDK)9 association with the AR. CDK9 phosphorylates the AR on S81 in vitro. Phosphorylation is specific to S81 because CDK9 did not phosphorylate the AR on other serine phosphorylation sites. Overexpression of CDK9 with its cognate cyclin, Cyclin T, increased S81 phosphorylation levels in cells. Small interfering RNA knockdown of CDK9 protein levels decreased hormone-induced S81 phosphorylation. Additionally, treatment of LNCaP cells with the CDK9 inhibitors, 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole and Flavopiridol, reduced S81 phosphorylation further, suggesting that CDK9 regulates S81 phosphorylation.

Anmerkungen Although nearly identical, nothing has been marked as a citation. It is not clear which work of Gioeli Rlm refers to. Is this vagueness intentional? Gioeli is one of the co-authors of Gordon et al 2010.

Sichter (Graf Isolan), SleepyHollow02

vorherige Seite | zur Übersichtsseite | folgende Seite
Letzte Bearbeitung dieser Seite: durch Benutzer:Singulus, Zeitstempel: 20141206201037