von Dott. Raffaele La Montagna
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
[1.] Rlm/Fragment 054 11 - Diskussion Zuletzt bearbeitet: 2014-12-05 19:02:52 Singulus | Fragment, Gesichtet, KomplettPlagiat, Rizzolio 2010, Rlm, SMWFragment, Schutzlevel sysop |
|
|
Untersuchte Arbeit: Seite: 54, Zeilen: 11-17 |
Quelle: Rizzolio 2010 Seite(n): 60, Zeilen: 12-18 |
---|---|
Lentiviral production
To generate knock down cells, lentiviral particles were produced as described (http://www.broadinstitute.org/genome_bio/trc/publicProtocols.html). Briefly, 1x106 293FT cells (Invitrogen Corp, Carlsbad, CA, USA) were transfected with 2.25 μg of PAX2 packaging plasmid, 0.75 μg of PMD2G envelop plasmid and 3μg of pLKO.1 hairpin vector utilizing [30 μl of Fugene HD (Roche Applied Science, Indianapolis, IN, USA) in 10 cm plate.] |
Lentiviral production
To generate knock down cells, lentiviral particles were produced as described (http://www.broadinstitute.org/genome_bio/trc/publicProtocols.html). Briefly, 1x106 293FT cells (Invitrogen Corp, Carlsbad, CA, USA) were transfected with 2.25 μg of PAX2 packaging plasmid, 0.75 μg of PMD2G envelop plasmid and 3μg of pLKO.1 hairpin vector utilizing 30 μl of Fugene HD (Roche Applied Science, Indianapolis, IN, USA) in 10 cm plate. |
Although identical, nothing has been marked as a citation. Continued on the next pages. |
|
Letzte Bearbeitung dieser Seite: durch Benutzer:Singulus, Zeitstempel: 20141205190337