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Typus
Verschleierung
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 37, Zeilen: 4-7, 9-15
Quelle: Rizzolio 2010
Seite(n): 48, Zeilen: 1-9
To assess the in vivo interaction between PIN1 and AR, LNCaP cells were immunoprecipitated with anti-PIN1 antibody and analyzed by western blot with anti-AR antibody. [...]

Co-immunoprecipitation is a purification procedure to determine if two different proteins interact. An antibody specific to the protein of interest is added to a cell lysate. Then the antibody-protein complex is pelleted usually using protein-A/G agarose, which binds most antibodies. If there are any proteins that bind to the first protein, they will also be pelleted. Identification of proteins in the pellet can be determined by western blot.

To assess the in vivo interaction between PIN1 and pRb, T98G cells were immunoprecipitated with anti-PIN1 antibody and analyzed by western blot with anti-pRb antibody.

Co-immunoprecipitation is a purification procedure to determine if two different proteins interact. An antibody specific to the protein of interest is added to a cell lysate. Then the antibody-protein complex is pelleted usually using protein-A/G agarose, which binds most antibodies. If there are any proteins that bind to the first protein, they will also be pelleted. Identification of proteins in the pellet can be determined by western blot.

Anmerkungen

Slightly adapted to the situation at hand, but otherwise identical. Nothing has been marked as a citation.

Sichter
(Graf Isolan), SleepyHollow02

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