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Membrane androgen receptor activation triggers pro-apoptotic responses in vitro and in vivo and blocks migration in colon cancer

von S. G.

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[1.] Shg/Fragment 042 01 - Diskussion
Zuletzt bearbeitet: 2014-11-02 01:24:59 Hindemith
Fragment, Gesichtet, Gu et al 2009, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Shg

Typus
KomplettPlagiat
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 42, Zeilen: 1-23
Quelle: Gu et al 2009
Seite(n): 4, Zeilen: li.Sp. 47-49 - re.Sp. 1-6, 12 ff.
At the age of 9 weeks animals were subjected to three cycles of alternating administration of distilled water containing 30 g/L synthetic dextran sulfate sodium (DSS; molecular mass 5000 Da; Wako Pure Chemical Industries, Led. Japan) for 7 days followed by distilled water for subsequent 14 days after intraperitoneal pretreatment with 20 mg/kg 1, 2-dimethylhydrazine (DMH; Sigma-Aldrich Corp. St.Louis.MO.USA). Group B mice received in addition to the carcinogenic treatment 5 mg/kg testosterone-HSA subcutaneously injected three times per week throughout the study period. All mice were sacrificed at the age of 20 weeks. After death, the entire colorectum from the colorectal junction to the anal verge was examined. Fresh specimens were placed in liquid nitrogen and subsequently stored at -80°C for further analysis. Then, the colon was opened longitudinally, washed with PBS, and divided into three portions (proximal, middle and distal). After macroscopic inspection the colon was fixed in a 40% g/L formaldehyde buffer solution (pH.7.4).

In APC mice, animal experiments were carried out in mice of either sex with mutated apc resulting in spontaneous colon tumor development (APCMin/+) obtained from the Jackson Laboratory (USA). The animals were housed under controlled environmental conditions (22-24°C, 50-70% humidity and a 12-h light/dark cycle). Throughout the study the mice had free access to standard pelleted food (C1000, Altromin, Lage, Germany) and tap water. All animal experiments were conducted according to the German law for the care and welfare of animals and were approved by local authorities.

Experiments were carried out on 7-week old wild-type Balb/c mice of either sex. The animals were housed under controlled environmental conditions (22-24°C, 50-70% humidity and a 12 h light/dark cycle). Throughout the study the mice had free access to standard pelleted food (C1310, Altromin, Heidenau, Germany) and tap water. All animal experiments were conducted according to the German law for the care and welfare of animals and were approved by local authorities

[...]

[...] At the age of 9 weeks animals were subjected to three cycles of alternating administration of distilled water containing 30 g/L synthetic dextran sulfate sodium (DSS; molelucar [sic] mass 5000 Da; Wako Pure Chemical Industries, Led. Japan) for 7 days followed by distilled water for subsequent 14 days after intraperitoneal pretreatment with 20 mg/kg 1, 2-dimethylhydrazine (DMH; Sigma-Aldrich Corp. St.Louis. MO. USA). Group B mice received in addition to the carcinogenic treatment 5 mg/kg testosterone-HSA subcutaneously injected three times per week throughout the study period. All mice were sacrificed at the age of 20 weeks. After death, the entire colorectum from the colorectal junction to the anal verge was examined. Fresh specimens were placed in liquid nitrogen and subsequently stored at -80°C for further analysis. Then, the colon was opened longitudinally, washed with PBS, and divided into three portions (proximal, middle and distal). After macroscopic inspection the colon was fixed in a 40% g/L formaldehyde buffer solution (pH.7.4).

Anmerkungen

Obwohl Shg als Coautor von Gu et al (2009) genannt wird, stammt keine der Formulierungen dieses Artikels von Shg (vgl. die Anmerkungen zu Quelle:Shg/Gu_et_al_2009).

Ergo: Übernahme eines Fremdtextes ohne jede Kennzeichnung.

Sichter
(Graf Isolan), SleepyHollow02


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