Fandom

VroniPlag Wiki

Shg/043

< Shg

31.377Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Teilen

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.

Membrane androgen receptor activation triggers pro-apoptotic responses in vitro and in vivo and blocks migration in colon cancer

von S. G.

vorherige Seite | zur Übersichtsseite | folgende Seite
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
[1.] Shg/Fragment 043 02 - Diskussion
Zuletzt bearbeitet: 2014-11-03 19:55:26 Graf Isolan
BauernOpfer, Fragment, Gesichtet, Gu et al 2009, SMWFragment, Schutzlevel sysop, Shg

Typus
BauernOpfer
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 43, Zeilen: 2-3, 11-20
Quelle: Gu et al 2009
Seite(n): 3, Zeilen: li. Sp. 11-12, (13-27), 27ff
3.2.4. Immunofluorescence analysis and confocal laser scanning microscopy

For testosterone-HSA-FITC staining, 5-μm-thick frozen tissue sections from the Balb/c or APC mouse tumors were fixed with 4% PFA for 15 min and incubated with 5% BSA/1x PBS/0.3% Triton for 1 hour at room temperature. After two washes with PBS 1.5% FBS specimens were exposed to testosterone-HSA-FITC (10-7 M, Sigma) for 1h at room temperature. Nuclei were stained with DRAQ-5 dye (1:1000, Biostatus, Leicestershire, UK) for 10 min at room temperature.

For direct fluorescence microscopy of F-actin, cells were fixed with 3 % paraformaldehyde in PBS for 30 min, permeabilized with 0.5 % Triton X-100 in PBS (10 min) and incubated with rhodamine-phalloidin (Molecular Probes, Eugene, OR, 1:100 dilution) for 40 min in the dark. For indirect immunofluorescence staining, cells were incubated for 2h at room temperature with mouse monoclonal anti-tubulin (Cell signaling, 1: 1000 dilution). Secondary FITC-conjugated rabbit anti-mouse IgG (Invitrogen) was used in a 1: 200 dilution. Nuclei were stained with DRAQ5™ (Biostatus Limited). Slides were mounted using the ProLang® Gold Antifade reagent (Invitrogen).

Immunofluorescence analysis and confocal laser scanning microscopy

Cells were cultured on glass cover slips with testosterone- HSA-FITC or control HSA-FITC using the concentrations and the incubation periods indicated in the figure legends. For testosterone-HSA-FITC staining, cells or specimens were washed twice with PBS containing 1.5% FBS for 1.5 min and incubated for 1 h with 1% BSA in PBS at room temperature. After two washes with PBS/1.5% FBS cells were exposed to 10-7 M testosterone-HSA-FITC, while control cells were incubated with 4 × 10-7 M HSA-FITC for 1 h at room temperature. Nuclei were stained with DRAQ5™ (Biostatus Limited) or TO-PRO-3 (Invitrogen). After two washes with PBS/1.5% FBS and fixation with 0.5% paraformaldehyde for 30 min cells were washed twice with PBS/1.5% FBS for 3 min and mounted with slow anti-fade. For direct fluorescence microscopy of F-actin, cells were fixed with 3% paraformaldehyde in PBS for 30 min, permeabilized with 0.5% Triton X-100 in PBS (10 min) and incubated with rhodamine-phalloidin (Molecular Probes, Eugene, OR, 1:100 dilution) for 40 min in the dark. For indirect immunofluorescence staining cells were incubated for 2 h at room temperature with mouse monoclonal anti-tubulin (Cell signaling, 1: 1000 dilution). Secondary FITC-conjugated rabbit anti-mouse IgG (Invitrogen) was used at a 1:200 dilution. Nuclei were stained with DRAQ5™ (Biostatus Limited). Slides were mounted using the ProLang® Gold Antifade reagent (Invitrogen).

Anmerkungen

Obwohl Shg als Coautor von Gu et al (2009) genannt wird, stammt keine der Formulierungen dieses Artikels von Shg (vgl. die Anmerkungen zu Quelle:Shg/Gu_et_al_2009).

Ergo: Insbesondere im zweiten Absatz liegt die Übernahme eines Fremdtextes ohne jede Kennzeichnung vor.

Ferner: aus dem "10-7" der Vorlage wird bei Shg "10-7".

Gu et al 2009 has been mentioned once on page 41 with respect only to "previous titration experiments".

Sichter
(SleepyHollow02), Graf Isolan


vorherige Seite | zur Übersichtsseite | folgende Seite
Letzte Bearbeitung dieser Seite: durch Benutzer:Graf Isolan, Zeitstempel: 20141103195641


Auch bei Fandom

Zufälliges Wiki