Fandom

VroniPlag Wiki

Shg/059

< Shg

31.359Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Share

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.

Membrane androgen receptor activation triggers pro-apoptotic responses in vitro and in vivo and blocks migration in colon cancer

von S. G.

vorherige Seite | zur Übersichtsseite | folgende Seite
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
[1.] Shg/Fragment 059 08 - Diskussion
Zuletzt bearbeitet: 2014-11-03 15:21:05 Hindemith
Fragment, Gesichtet, Gu et al 2009, SMWFragment, Schutzlevel sysop, Shg, Verschleierung

Typus
Verschleierung
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 59, Zeilen: 8-26
Quelle: Gu et al 2009
Seite(n): 6, 7, Zeilen: 6: l.col: 3ff; 7: r.col: 1ff
4.6 mAR activation triggered rapid actin and tubulin reorganization in colon cancer cells

Cytoskeleton reorganization is a prominent early functional response of various cancer cells to steroid hormones targeting membrane binding sites [Koukouritaki et al., 1997, Kampa et al., 2002, Kampa et al., 2006, Papadopoulou et al., 2008a]. Accordingly, to analyze the functional impact of mAR in colon cancer rapid cytoskeleton modifications was investigated in Caco2 cells upon activation of mAR with testosterone-HSA for various time intervals. Cellular actin cytoskeleton dynamics were initially assessed by appropriate quantitative techniques as described in Papakonstanti et al., 2007. As shown in figure 13A, quantitative immunoblot analysis of Triton X-100 insoluble cytoskeletal pellets and corresponding supernatants revealed a significant decrease of the Triton-soluble (monomeric) to total actin ratio in Caco2 cells treated with 10-7 M testosterone-HAS, indicating actin polymerization. This effect was evident 15 min upon testosterone-HSA treatment; and returned to nearly control levels after 1-2 hours (Fig. 13A). The quantitative data were fully supported by confocal laser scanning microscopic analysis, showing redistribution of microfilamentous structures and formation of stress fibers and filopodia in testosterone-HSA treated cells (Fig. 13B).


Kampa M, Papakonstanti EA, Hatzoglou A, Stathopoulos EN, Stournaras C, Castanas E. (2002). The human prostate cancer cell line LNCaP bears functional membrane testosterone receptors that increase PSA secretion and modify actin cytoskeleton. Faseb J, 16:1429-1431.

Kampa M, Kogia C, Theodoropoulos PA, Anezinis P, Charalampopoulos I, Papakonstanti EA, Stathopoulos EN, Hatzoglou A, Stournaras C, Gravanis A, Castanas E. (2006) Activation of membrane androgen receptors potentiates the antiproliferative effects of paclitaxel on human prostate cancer cells. Mol Cancer Ther, 5:1342-1351.

Papadopoulou N, Charalampopoulos I, Alevizopoulos K, Gravanis A, Stournaras C. (2008 a). Rho/ROCK/Actin signaling regualtes membrane androgen receptor induced apoptosis in prostate cancer cells. Exp Cell Res, 314: 3162-3174.

mAR activation triggered rapid actin and tubulin reorganization in colon cancer cells

Cytoskeleton reorganization is a prominent early functional response of various cancer cells to steroid hormones targeting membrane binding sites [3,8,19,27]. Accordingly to analyze the functional impact of mAR in colon cancer we investigated rapid cytoskeleton modifications in Caco2 cells upon activation of mAR with testosterone- HSA for various time intervals. Cellular actin cytoskeleton dynamics were initially assessed by appropriate quantitative techniques as described in [25]. As shown in fig. 3A, quantitative immunoblot analysis of Triton X-100-insoluble cytoskeletal pellets and correspond-

[Seite 7]

ing supernatants revealed a significant decrease of the Triton-soluble (monomeric) over total actin ratio in Caco2 cells treated with 10-7 M testosterone-HSA, indicating actin polymerization. This effect was evident 15 min upon testosterone-HSA treatment and returned to nearly control levels after 1-2 h (Fig. 3A). The quantitative data were fully supported by confocal laser scanning microscopic analysis showing redistribution of microfilamentous structures and formation of stress fibers and filopodia in testosterone-HSA treated cells (Fig. 3B).


3. Kampa M, Papakonstanti EA, Hatzoglou A, Stathopoulos EN, Stournaras C, Castanas E: The human prostate cancer cell line LNCaP bears functional membrane testosterone receptors that increase PSA secretion and modify actin cytoskeleton. Faseb J 2002, 16:1429-1431.

8. Papadopoulou N, Charalampopoulos I, Alevizopoulos K, Gravanis A, Stournaras C: Rho/ROCK/Actin signaling regualtes membrane androgen receptor induced apoptosis in prostate cancer cells. Exp Cell Res 2008, 314:3162-3174.

19. Kampa M, Kogia C, Theodoropoulos PA, Anezinis P, Charalampopoulos I, Papakonstanti EA, Stathopoulos EN, Hatzoglou A, Stournaras C, Gravanis A, Castanas E: Activation of membrane androgen receptors potentiates the antiproliferative effects of paclitaxel on human prostate cancer cells. Mol Cancer Ther 2006, 5:1342-1351.

27. Koukouritaki S, Margioris A, Gravanis A, Hartig R, Stournaras C: Dexamethasone induces actin polymerization in human endometrial cells without affecting its synthesis. J Cell Biochem 1997, 65:492-500

Anmerkungen

Die Quelle Koukouritaki fehlt im Literaturverzeichnis.

Man beachte die Anmerkung zur Quelle Gu et al. (2009)

Sichter
(SleepyHollow02), Hindemith


vorherige Seite | zur Übersichtsseite | folgende Seite
Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20141103152146

Auch bei Fandom

Zufälliges Wiki