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BauernOpfer
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SleepyHollow02
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Untersuchte Arbeit:
Seite: 51, Zeilen: 1-9
Quelle: Gu et al 2009
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Confocal laser scanning microscopic analysis of Caco2 cells (a-d) and HCT 116 cells(e-h) stained with testosterone-HSA-FITC, showing specific FITC related fluorescence at the cell membranes, or HSA-FITC, showing no apparent membrane staining. Visualization of nuclei was evident by DRAQ5™ or TO-PRO-3 staining. Magnification, ×100.

A

B

Figure 6 Membrane staining of mAR in IEC 06 cells and iAR silenced Caco2 cells. A) Confocal laser scanning microscopic analysis of IEC 06 cells (a-b) stained with testosterone-HSA-FITC, showing no apparent membrane fluorescence at the cell membrane. Visualization of nuclei was evident by DRAQ5™ staining. B) Confocal laser scanning microscopic analysis of iAR silenced cells (a-d) stained with testosterone-HSA-FITC, showing membrane fluorescence at the cell membrane. Visualization of nuclei was evident by DRAQ5™ staining.

Membrane staining of mAR in colon tumors and colon cancer cells. Confocal laser scanning microscopic analysis of WCL2 (a-c) and MCL2 (d-f) colon tumor specimens and HCT116 cells (g-j) stained with testosterone-HSA-FITC, showing specific FITC related fluorescence at the cell membranes. Control staining with HSA-FITC showed no apparent membrane fluorescence. Visualization of nuclei was evident by DAPI or TO-PRO-3 staining. Magnification, ×100.

Membrane staining and binding assays of mAR in Caco-2 cells. A) Confocal laser scanning microscopic analysis of Caco2 cells (a-d) stained with testosterone-HSA-FITC, showing specific FITC related fluorescence at the cell membranes (a, b). No apparent membrane fluorescence was shown in control samples stained with HSA-FITC (c, d). Visualization of nuclei was evident by DRAQ5™ staining. Magnification, ×100. B) Confocal laser scanning microscopic analysis of IEC 06 cells (e-f) stained with testosterone-HSA-FITC, showing no apparent membrane fluorescence at the cell membrane. Visualization of nuclei was evident by DRAQ5™ staining.

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