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SleepyHollow02
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Untersuchte Arbeit:
Seite: 66, Zeilen: 1-9
Quelle: Gu et al 2009
Seite(n): 0, Zeilen: 0
Figure 19: Pro-apoptotic effects of testosterone-HSA in the absence or presence of inhibitors in Caco2 cells

Quantitative APOPercentage apoptosis assay of TAC stimulated Caco2 cells and similar experiments in the presence of anastrozole. Cells were exposed or not to 10-7 M testosterone-HSA for 24 hours and proapoptoric responses were assessed by the APOPercentage apoptosis assay. Equally, cells pre-treated or not with 10-6 M anastrozole was exposed to testosterone-HSA for 24 hours. Cells serum starved for comparable periods of time served as a positive control for apoptosis. Bars present the mean OD measured at 550 nm. *** P<0,001, n=3.

Thus, we aimed to further evaluate the in vivo effects of albumin- PFrigou-arpeo p5totic effects of testosterone-HSA, DHT and estradiol in the absence or presence of inhibitors in Caco2 cells Pro-apoptotic effects of testosterone-HSA, DHT and estradiol in the absence or presence of inhibitors in Caco2 cells. (A) Quantitative APOPercentage apoptosis assay of testosterone-HSA, DHT or estradiol stimulated Caco2 cells and in the presence/absence of cytochalasin B (Cyto B) or flutamide. Cells were exposed to 10-7 M testosterone-HSA, DHT or estradiol for 24 hours and pro-apoptoric responses were assessed by the APOPercentage apoptosis assay. Equally, cells pretreated or not with 10-7 M Cyto B or flutamide, were exposed to testosterone-HSA for 24 hours. Cells serum starved for comparable periods of time served as a positive control for apoptosis. Bars present the mean OD measured at 550 nm (** P < 0.01, N = 4). (B) Cells were pre-treated or not with Cyto B or flutamide for 1 h and then exposed or not to 10-7 M testosterone- HSA for 4 h, lysed and incubated with the caspase-3 substrate DEVD conjugated to the chromophore pNA according to the manufacturer's instructions.
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