Fandom

VroniPlag Wiki

Sj/Dublette/Fragment 024 01

< Sj

31.268Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Share

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.


Typus
KeineWertung
Bearbeiter
Graf Isolan
Gesichtet
No.png
Untersuchte Arbeit:
Seite: 24, Zeilen: 1-15
Quelle: Pan 1996
Seite(n): 17-18, Zeilen: 17:13-20 - 18:1ff
3.3 Xenopus laevis oocytes

One of the first and still most widely used assay system for quantifying an authentic protein biosynthetic process is the fully grown oocyte of the South African clawed frog, Xenopus laevis. The value of Xenopus laevis first became apparent in 1971, when Gurdon and co-workers discovered that the oocyte constitutes an efficient system for translating foreign messenger RNA.

The Xenopus oocyte is a cell specialized for the production and storage of proteins for later use during embryogenesis and developmentally divided into 6 stages (112). In addition, the complex architecture of the frog oocyte includes the subcellular systems involved in the export and import of proteins. Therefore, the mRNA-microinjected oocyte is an appropriate system to study the synthesis of specific polypeptides, as well as the storage of particular proteins in various subcellular organelles and the export of others into the extracellular space. Moreover, the subcellular compartmentalization, as well as the structure and biochemical, physiological, and biological properties of the synthesized protein, may be examined from exogenous proteins in the injected oocyte.


112. Costa,PF, Emilio,MG, Fernandes,PL, Ferreira,HG, Ferreira,KG: Determination of ionic permeability coefficients of the plasma membrane of Xenopus laevis oocytes under voltage clamp. J.Physiol 413:199-211, 1989

[Page 17]

Use of Xenopus Oocytes as a Model System. One of the first and still most widely used assay system for quantifying an authentic protein biosynthetic processes is the fully grown oocyte of the South African clawed frog, Xenopus laevis. The value of Xenopus laevis first became apparent in 1971 when Gurdon and co-workers discovered that the oocyte constitutes an efficient system for translating foreign messenger RNA (Gurdon et al., 1971).

The Xenopus oocyte is a cell specialized for the production and storage of proteins for later use during embryogenesis. In addition, the complex architecture of the

[Page 18]

frog oocyte includes the subcellular systems involved in the export and import of proteins. Therefore, the mRNA-microinjected oocyte is an appropriate system in which to study the synthesis of specific polypeptides, as well as the storage of particular proteins in various subcellular organelles and the export of others into the extracellular space. Moreover the subcellular compartmentalization, as well as the structure and biochemical, physiological, and biological properties of the synthesized protein, may be examined in the injected oocyte.


Gurdon, J. B., C. D. Lane, H. R. Woodland, and G. Marbaix. 1971. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature. 233: 177-182.

Anmerkungen

Nothing has been marked as a citation.

Sichter
(Graf Isolan)

Auch bei Fandom

Zufälliges Wiki