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Typus
KomplettPlagiat
Bearbeiter
Hindemith
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 25, Zeilen: 1-9
Quelle: Embark 2004
Seite(n): 32, Zeilen: 4ff
[However, in NaPi-3 expressing Xenopus oocytes PKC-mediated PTH regulation] can not be observed. Instead, coupling to the PKA pathway leads to the alteration of PKA-regulated ion channels (114). Exposing the Xenopus oocytes to the regulators of intracellular signaling such as PKC activator phorbol esters may unspecifically lead to internalization of the plasma membrane and the expressed proteins (115;116). In summary, the Xenopus oocyte system has the advantage that channels, receptors and transporters can rapidly be expressed and identified by their electrophysiological properties. Once cDNA clones have been isolated, oocytes are an excellent system for correlating structure with function using a combination of molecular biological and electrophysiological techniques and analyzed both biochemically and electro physiologically in an in vivo situation.

114. Waldegger,S, Raber,G, Sussbrich,H, Ruppersberg,JP, Fakler,B, Murer,H, Lang,F, Busch,AE: Coexpression and stimulation of parathyroid hormone receptor positively regulates slowly activating IsK channels expressed in Xenopus oocytes. Kidney Int. 49:112-116, 1996

115. Vasilets,LA, Schwarz,W: Regulation of endogenous and expressed Na+/K+ pumps in Xenopus oocytes by membrane potential and stimulation of protein kinases. J.Membr.Biol. 125:119-132, 1992

116. Loo,DD, Hirsch,JR, Sarkar,HK, Wright,EM: Regulation of the mouse retinal taurine transporter (TAUT) by protein kinases in Xenopus oocytes. FEBS Lett. 392:250-254, 1996

However, in NaPi-3 expressing Xenopus oocytes PKC-mediated PTH regulation can not be observed (Wagner et al., 1996). Instead, coupling to the PKA pathway leads to the alteration of PKA-regulated ion channels (Waldegger et al., 1996). Exposing the Xenopus oocytes to the regulators of intracellular signaling such as PKC activator phorbol esters may unspecifically lead to internalization of the plasma membrane and the expressed proteins (Vasilets and Schwarz, 1992; Loo et al., 1996).

In summary, the Xenopus oocyte system has the advantage that channels, receptors and transporters can rapidly be expressed and analyzed both biochemically and electrophysiologically in an in vivo situation. The system can be used quite effectively as an assay for the functional cloning of channels that have only been identified by their electrophysiological properties. Once cDNA clones have been isolated, oocytes are an excellent system for correlating structure with function using a combination of molecular biological and electrophysiological techniques.

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Sichter
(Hindemith) LieschenMueller

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