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[1.] Sng/Fragment 046 01 - Diskussion
Zuletzt bearbeitet: 2016-05-22 21:37:56 Schumann
Fragment, Gesichtet, KomplettPlagiat, SMWFragment, Sajikumar 2005, Schutzlevel sysop, Sng

Typus
KomplettPlagiat
Bearbeiter
SleepyHollow02, Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 46, Zeilen: 1-21
Quelle: Sajikumar 2005
Seite(n): 44-45, Zeilen: 44:7-25, 45:1-9
[Knowing the metabolic instability during that period we prolonged the preincubation of hippocampal slices to at least 4 h to obtain comparable] and more physiological results in describing functional processes in slice preparations. This requirement is supported by additional data such as measuring basal endogenous protein phosphorylation patterns and the translocation of different protein kinase C isoenzymes (α, β and γ) to the membrane as markers of their activation in tissue obtained from hippocampal slices in vitro or from intact, untreated rats. Studies revealed that only slices incubated in the same way as described here showed comparable patterns of phosphorylation and enzyme translocation as detected in the intact animal (Angenstein and Staak, 1997). Although one could argue that specific modifications of slice preparation may circumvent distinct problems raised above, to maintain the complex slice physiology at a level which allows reliable studies of functional plasticity favours a more simple method: to wait (Sajikumar and Frey, 2004a).

Following the preincubation period, the test stimulation strength was determined for each input to elicit a population spike of about 40 % (for LTD studies) or 25 % (for studies conducted to investigate LTP) of its maximal amplitude determined by slice specific input-output relationship. For stimulation, biphasic constant current pulses were used. The baseline was recorded for at least 60 min before LTP/LTD induction. Four 0.2 Hz biphasic, constant-current pulses (0.1 ms per polarity) were used for testing 1, 3, 5, 11, 15, 21, 25, 30 min post-tetanus or 21, 25, 30 min post-LFS and thereafter once every 15 min up to 8 h. Since the two recorded parameters showed either similar time course in the experiments (if the population spike was not abolished after induction of LTD at all), for clarity only the fEPSP data are shown. A detailed description of the experimental protocol for the preparation, incubation and investigation of reliable rat hippocampal CA1 late-LTP/LTD is shown in Fig. 5 (Sajikumar et al., 2005a).


13. Angenstein F, Staak S (1997) Receptor-mediated activation of protein kinase C in hippocampal long-term potentiation: facts, problems and implications. Prog Neuropsychopharmacol Biol Psychiatry 21: 427-454.

137. Sajikumar S, Frey JU (2004a) Late-associativity, synaptic tagging, and the role of dopamine during LTP and LTD. Neurobiol Learn Mem 82: 12-25.

139. Sajikumar S, Navakkode S, Frey JU (2005a) Protein synthesis-dependent long-term functional plasticity: methods and techniques. Curr Opin Neurobiol ..

[Seite 44]

Knowing the metabolic instability during that period we prolonged the preincubation of hippocampal slices to at least 4 h to obtain comparable and more physiological results in describing functional processes in slice preparations. This requirement is supported by additional data such as measuring basal endogenous protein phosphorylation patterns and the translocation of different protein kinase C isoenzymes (α, β and γ) to the membrane as markers of their activation in tissue obtained from hippocampal slices in vitro or from intact, untreated rats. Studies revealed that only slices incubated in the same way as described here showed comparable patterns of phosphorylation and enzyme translocation as detected in the intact animal (Angenstein and Staak, 1997). Although one could argue that specific modifications of slice preparation may circumvent distinct problems raised above, to maintain the complex slice physiology at a level which allows reliable studies of functional plasticity favors a more simple method: to wait (Sajikumar and Frey, 2004a).

Following the preincubation period, the test stimulation strength was determined for each input to elicit a population spike of about 40 % (for LTD studies) or 25 % (for studies conducted to investigate LTP and the effect of dopamine application) of its maximal amplitude determined by slice specific

[Seite 45]

input-output relationship. For stimulation, biphasic constant current pulses were used. The baseline was recorded for at least 60 min before LTP/LTD induction. In the dopamine studies the baseline was recorded for at least 30 min. Four 0.2 Hz biphasic, constant-current pulses (0.1 ms per polarity) were used for testing 1, 3, 5, 11, 15, 21, 25, 30 min post-tetanus or 21, 25, 30 min post-LFS and thereafter once every 15 min up to 8 h (30 min in dopamine series). Since the two recorded parameters showed either similar time course in the experiments (if the population spike was not abolished after induction of LTD at all), for clarity only the fEPSP data are shown.


Angenstein F, Staak S (1997) Receptor-mediated activation of protein kinase C in hippocampal long-term potentiation: facts, problems and implications. Prog Neuropsychopharmacol Biol Psychiatry 21: 427-454.

Sajikumar S, Frey JU (2004a) Late-associativity, synaptic tagging, and the role of dopamine during LTP and LTD. Neurobiol Learn Mem 82: 12-25.

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