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Typus
Verschleierung
Bearbeiter
Graf Isolan
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 42, Zeilen: 1-10, 12-21
Quelle: Sajikumar 2005
Seite(n): 40, Zeilen: 1-19
2. Materials and methods

2.1. Hippocampal slice preparation

All experiments were performed in right hippocampal slices (400 μm thick) prepared from 7 weeks old male Wistar rats (total number of animals: 310). The animal was stunned by a blow behind the foramen magnum and decapitated (cervical dislocation). Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and dura removed before transferring the brain into chilled and carbogenated (carbogen: gas consisting of 95% O2 and 5% CO2) artificial cerebrospinal fluid (ACSF) (about 4°C) (Reymann et al., 1985). [...] Divide the remaining part of the brain in the central sulcus by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of manuel tissue chopper (Cambden, UK), and 400 μm thick slices were cut at 70° transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush, floated in a petri dish containing the cooled and carbogenated ACSF, and immediately transferred to the nylon net in the experimental chamber maintained at 32°C by a wide bored pipette. One of the critical points which elapse between the removal of the brain and the placing of the slices in the chamber is that slice preparation should be performed in less than 3 min [and favourably at a temperature of 4°C to minimize cellular metabolism and to avoid irreversible intracellular phase changes.]


133. Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15: 249-255.

2.0. Materials and methods

2.1. Brain slice preparation and incubation

All experiments were performed in right hippocampal slices (400 μm thick) prepared from 7 weeks old male Wistar rats (total number of animals: 275). The animal was stunned by a blow behind the foramen magnum and decapitated immediately. Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and dura removed before transfering the brain into cooled and carbogenated (carbogen: gas consisiting [sic!] of 95% O2 and 5% CO2) artifical cerebro spinal fluid (ACSF) (about 4°C). Cold solution was used to slow down the metabolism of the tissue, to limit the extent of excitotoxic and other kinds of damage occurring during the preparation of slices (Reymann et al., 1985). The hemispheres were separated mid-sagitally by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of McIIwain tissue chopper (Cambden,UK), and 400 μm slices were cut at 70° transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush, floated in a petri dish containing the cooled and carbogenated ACSF, and immediately transfered to the nylon net in the experimental chamber by a wide bored pipette. One of the critical points which elapses between the removal of the brain and the placing of the slices in the chamber, is that time should not exceed 4 min.


Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15: 249-255.

Anmerkungen

Im Wortlaut übereinstimmend. Ohne Hinweis auf eine Übernahme.

Sichter
(Graf Isolan) Agrippina1

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