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| Untersuchte Arbeit: Seite: 43, Zeilen: 10-20 |
Quelle: Sajikumar 2005 Seite(n): 40-41, 42, Zeilen: 40:24-25 - 41:1-6; 42:10-13 |
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| When slices are taken out with proper care the responses, observed on stimulation are similar to those seen in intact animals. Slices were incubated within an interface chamber (Fig. 4) at 32°C (carbogenated incubation medium contained 124 mM NaCl, 4.9 mM KCl, 1.2 mM KH2PO4, 2.0 mM MgSO4, 2.0 mM CaCl2, 24.6mM NaHCO3, 10 mM D-glucose). Supply of oxygen was achieved by controlling the gas flow over the surface of the slice (carbogen flow rate: 32 l/h) thus preventing the drying out of the slices (Sajikumar et al., 2005a).
Slices were preincubated for at least 4 h, a quite unusual long period, but it has been shown by the following reasons to be critical for a stable long-term recording as well as the study of late plasticity for up to 16 h, under conditions which resemble the functionality of studies in vivo. 139. Sajikumar S, Navakkode S, Frey JU (2005a) Protein synthesis-dependent long-term functional plasticity: methods and techniques. Curr Opin Neurobiol .. |
[Seite 40]
When slices are taken out with proper care the responses, observed on stimulation are similar [Seite 41] to those seen in intact animals. Slices were incubated within an interface chamber at 32°C (carbogenated incubation medium contained 124 mM NaCl, 4.9 mM KCl, 1.2 mM KH2PO4, 2.0 mM MgSO4, 2.0 mM CaCl2, 24.6mM NaHCO3, 10 mM D-glucose). Supply of oxygen was achieved by controlling the gas flow over the surface of the slice (carbogen flow rate: 18 l/h) thus preventing the drying out of the slices (see Fig. 3). [Seite 42] Slices were preincubated for at least 4 h, a quite unusual long period, but it has been shown by the following reasons to be critical for a stable long-term recording as well as the study of late plasticity for up to 16 h, under conditions which resemble the functionality of studies in vivo. |
Art und Umfang der Übernahme bleiben ungekennzeichnet. Die Nennung eines Artikels, welcher auf der Arbeit Sajikumar (2005) basiert, führt zur Einstufung als "BauernOpfer". |
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