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Identification and characterization of Fluorescent Protein from marine organisms and potentially applications

von Dr. Tiziana Masullo

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[1.] Tim/Fragment 019 01 - Diskussion
Zuletzt bearbeitet: 2014-10-15 18:50:19 Singulus
Chapleau 2008, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Tim, Verschleierung

Typus
Verschleierung
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 19, Zeilen: 1-2
Quelle: Chapleau 2008
Seite(n): 619, Zeilen: l.col.: 14 ff.
Previous studies have suggested that the irregular conformation of the structure might allow solutes to penetrate into the core of the protein (Agmon, 2005). Previous studies have suggested that the irregular conformation of the structure might allow solutes to penetrate into the core of the protein (Agmon 2005).
Anmerkungen
Sichter
(SleepyHollow02) Singulus

[2.] Tim/Fragment 019 03 - Diskussion
Zuletzt bearbeitet: 2014-11-27 22:12:51 Hindemith
BauernOpfer, Fragment, Gesichtet, Isarankura 2009, SMWFragment, Schutzlevel sysop, Tim

Typus
BauernOpfer
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 19, Zeilen: 3-16
Quelle: Isarankura 2009
Seite(n): 352, Zeilen: abstract
Isarankura et al. (2009) explored spectroscopic determinations of copper ions using chimeric metal-binding green fluorescent protein His6GFP (espressed in E. coli and purified to homogeneity) as an active indicator. Supplementation of copper ions to the GFP solution led to a remarkable decrease of fluorescent intensity corresponding to metal concentrations. For circumstances, rapid declining of fluorescence up to 60% was detected in the presence of 500 μM copper. This was in contrast to those observed in the case of zinc and calcium ions, in which approximately 10–20% of fluorescence was affected (fig. 11). Recovery of its original fluorescence up to 80% was mediated by the addition of EDTA.

More importantly, in the presence of metal ions, the emission wavelength maximum remained unchanged while reduction of the optical density of the absorption spectrum has been observed. This indicated that the chromophore‟s ground state was possibly affected by the static quenching process rather than structural or conformational alteration.

Abstract

In the present study, spectroscopic determinations of copper ions using chimeric metal-binding green fluorescent protein (His6GFP) as an active indicator have been explored. Supplementation of copper ions to the GFP solution led to a remarkable decrease of fluorescent intensity corresponding to metal concentrations. For circumstances, rapid declining of fluorescence up to 60% was detected in the presence of 500 μM copper. This is in contrast to those observed in the case of zinc and calcium ions, in which approximately 10–20% of fluorescence was affected. Recovery of its original fluorescence up to 80% was mediated by the addition of ethylenediamine tetraacetic acid. More importantly, in the presence of metal ions, the emission wavelength maximum remains unchanged while reduction of the optical density of the absorption spectrum has been observed. This indicates that the chromophore’s ground state was possibly affected by the static quenching process.

Anmerkungen

The source is given, but it is not clear to the reader that the following text is taken from it. The copied text includes, what could be understood as an interpretation / evaluation of the results of the source: "a remarkable decrease", "More importantly", "This indicated that", "possibly".

Sichter
(SleepyHollow02), Hindemith


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