Fandom

VroniPlag Wiki

Tim/020

< Tim

31.371Seiten in
diesem Wiki
Seite hinzufügen
Diskussion0 Teilen

Störung durch Adblocker erkannt!


Wikia ist eine gebührenfreie Seite, die sich durch Werbung finanziert. Benutzer, die Adblocker einsetzen, haben eine modifizierte Ansicht der Seite.

Wikia ist nicht verfügbar, wenn du weitere Modifikationen in dem Adblocker-Programm gemacht hast. Wenn du sie entfernst, dann wird die Seite ohne Probleme geladen.

Identification and characterization of Fluorescent Protein from marine organisms and potentially applications

von Dr. Tiziana Masullo

vorherige Seite | zur Übersichtsseite | folgende Seite
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
[1.] Tim/Fragment 020 01 - Diskussion
Zuletzt bearbeitet: 2014-11-27 22:14:41 Hindemith
BauernOpfer, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Sumner et al 2006, Tim

Typus
BauernOpfer
Bearbeiter
Hindemith
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 20, Zeilen: 1-15
Quelle: Sumner et al 2006
Seite(n): 1303, 1306, Zeilen: 1303: l.col: 25 ff.; 1305: l.col: 21 ff.; 1306: r.col: 7 ff.
Sumner et al. (2006) described the quenching of the fluorescence of DsRed (Fradkov et al., 2002) by Cu+ and Cu2+. They showed that the DsRed tetramer can be used as a sensitive fluorescent probe towards both monovalent and divalent copper ions, even in the presence of competing metal ions, with a detection limit well below 1 ppb. The wild type DsRed is 40 times more sensitive than the engineered GFPs and seven orders of magnitude more sensitive to Cu2+ ions than the wild type GFP. Even more impressive is the ability of DsRed to bind Cu+ with nanomolar sensitivity and a fast response time. They for first demonstrated that the addiction of Mn2+, Fe2+/3+, Co2+, Ni2+, Zn2+, Cd2+, Ag+, Hg2+, Pb2+, Mg2+ or Ca2+ did not competed for the copper binding site and did not interfered under these conditions. These data also indicated that the quenching was not a result of collisional quenching. With EDTA the fluorescence intensity returned to 90% of its initial value. Copper ions prefer coordination with amines, ionized peptide nitrogens and thiolates (Silvia and Williams, 1991); structural motifs that have high affinity for Cu2+ are frequently composed of the side chains of His, Tyr, Glu, Asp, Cys, and sometimes Asn and Gln (Sigel and Martin, 1982). DsRed contains a single cysteine [which is solvent accessible and many solvent accessible His, Glu, and Asp, as shown by the X-ray structure (Wall et al., 2000).] In this report, we describe the quenching of the intrinsic fluorescence of DsRed (Fradkov et al., 2002) by Cu+ and Cu2+; [...] Here we show that the DsRed tetramer can be used as a sensitive fluorescent probe towards both monovalent and divalent copper ions, even in the presence of competing metal ions, with a detection limit well below 1 ppb. The wild type DsRed is 40 times more sensitive than the engineered GFPs and seven orders of magnitude more sensitive to Cu2+ ions than the wild type GFP. Even more impressive is the ability of DsRed to bind Cu+ with nanomolar sensitivity and a fast response time.

[page 1305]

As an initial test of the selectivity, we first demonstrated that the DsRed fluorescence is unaffected by the addition of 1μM of Mn2+, Fe2+/3+, Co2+, Ni2+, Zn2+, Cd2+, Ag+, Hg2+, Pb2+, or 1μM Mg2+ or Ca2+. [...] Furthermore, other transition metals do not affect the Cu2+-mediated quenching of DsRed (Fig. 3, front row), indicating that these metals do not compete for the copper binding site and do not interfere under these conditions. [...]

[...] The fluorescence intensity, after being quenched by copper(II), returns to 90% of its initial value within 2 min when EDTA, a metal ion chelator, is added and allowed to equilibrate.

[page 1306]

Copper ions prefer coordination with amines, ionized peptide nitrogens and thiolates (Silvia and Williams, 1991); structural motifs that have high affinity for Cu2+ are frequently composed of the side chains of His, Tyr, Glu, Asp, Cys, and sometimes Asn and Gln (Sigel and Martin, 1982) and yield square planar, trigonal bipyramid and octahedral geometries. DsRed contains a single cysteine which is solvent accessible and many solvent accessible His, Glu, and Asp, as shown by the X-ray structure (Wall et al., 2000).

Anmerkungen

The source is mentioned in the beginning, but without making clear that the whole page is taken from it largely literally. In the beginning of the fragment the work of Sumner et al. (2006) is described and the reader may expect a certain closeness, but starting with "Copper ions prefer" findings of other papers are described and the reader cannot assume in any way that this passage is also a literal copy of Sumner et al. (2006).

Sichter
(Hindemith), SleepyHollow02


vorherige Seite | zur Übersichtsseite | folgende Seite
Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20141127221730

Auch bei Fandom

Zufälliges Wiki