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Identification and characterization of Fluorescent Protein from marine organisms and potentially applications

von Dr. Tiziana Masullo

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[1.] Tim/Fragment 023 03 - Diskussion
Zuletzt bearbeitet: 2014-10-27 04:52:36 Hindemith
Fragment, Gesichtet, Isarankura 2009, SMWFragment, Schutzlevel sysop, Tim, Verschleierung

Typus
Verschleierung
Bearbeiter
SleepyHollow02
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 23, Zeilen: 3-5
Quelle: Isarankura 2009
Seite(n): 353, Zeilen: last lines
Engineered E. coli co-expressing surface exposed zinc-binding motif and GFP has been applied for real-time monitoring of intracellular mobility of zinc ions (Isarankura-Na-Ayudhya et al., 2005).

Isarankura-Na-Ayudhya C., Suwanwong Y., Boonpangrak S., Kiatfuengfoo R., Prachayasittikul V., 2005. Co-expression of zinc binding motif and GFP as a cellular indicator of metal ions mobility. Int. J. Biol. Sci., 1: 146-151.

Additionally, engineered E. coli co-expressing surface exposed zinc-binding motif and GFP has been applied for real-time monitoring of intracellular mobility of zinc ions [27].

27. Isarankura-Na-Ayudhya C, Suwanwong Y, Boonpangrak S et al (2005) Co-expression of zinc binding motif and GFP as a cellular indicator of metal ions mobility. Int J Biol Sci 1:146–151

Anmerkungen

The source given does not contain the copied text.

Sichter
(SleepyHollow02), Hindemith

[2.] Tim/Fragment 023 19 - Diskussion
Zuletzt bearbeitet: 2014-10-27 04:13:04 Hindemith
BauernOpfer, Fragment, Fu 2008, Gesichtet, SMWFragment, Schutzlevel sysop, Tim

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SleepyHollow02
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Untersuchte Arbeit:
Seite: 23, Zeilen: 19-26
Quelle: Fu 2008
Seite(n): abstract, p.6, Zeilen: p.6: last lines
In the study of 2008, Fu et al. observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking. The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection. In this report, we observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking.

The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection.

Anmerkungen

The source is given, but it is not clear to the reader how much has been taken from it. In particular the two sentences starting with "Accordingly, we observed" cannot be attributed to the source by the reader who rather assumes that the author herself made observations that are consistent with the findings of the source.

Sichter
(SleepyHollow02), Hindemith


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