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| Untersuchte Arbeit: Seite: 8, Zeilen: 1-7 |
Quelle: Nienhaus 2006 Seite(n): 4213, Zeilen: l.col: 1 ff. |
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| Figure 2 C displays the chromophore cage, with potential hydrogen-bond interactions represented by dashed lines. The highly conserved residues Arg92 and Glu212 have been implicated as being crucially involved in the mechanism of autocatalytic chromophore formation (Ormo et al., 1996; Sniegowski et al., 2005; Wood et al., 2005). The Arg92 guanidinium group hydrogen bonds to the Tyr64-derived carbonyl oxygen, whereas Glu212 is positioned within hydrogen-bonding distance to the heterocyclic ring nitrogen. | Fig. 1 C displays the chromophore cage, with potential hydrogen-bond interactions represented by dashed lines. [...] The highly conserved residues Arg92 and Glu212 have been implicated as being crucially involved in the mechanism of autocatalytic chromophore formation (6,40,41). The Arg92 guanidinium group hydrogen bonds to the Tyr64-derived carbonyl oxygen, whereas Glu212 is positioned within hydrogen-bonding distance to the heterocyclic ring nitrogen, as has been observed earlier for yellow avGFP variants (42)
6. Ormo¨ , M., A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 273:1392–1395. 40. Sniegowski, J. A., J. W. Lappe, H. N. Patel, H. A. Huffman, and R. M. Wachter. 2005. Base catalysis of chromophore formation in Arg96 and Glu222 variants of green fluorescent protein. J. Biol. Chem. 280: 26248–26255. 41. Wood, T. I., D. P. Barondeau, C. Hitomi, C. J. Kassmann, J. A. Tainer, and E. D. Getzoff. 2005. Defining the role of arginine 96 in green fluorescent protein fluorophore biosynthesis. Biochemistry. 44:16211– 16220. 42. Wachter, R. M., M. A. Elsliger, K. Kallio, G. T. Hanson, and S. J. Remington. 1998. Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure. 6: 1267–1277. |
The source is given the previous page to reference the figure that is described here. Nothing indicates, however, that this section is also taken from the source. |
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