# Tim/Fragment 008 10

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 Typus BauernOpfer Bearbeiter Hindemith Gesichtet
Untersuchte Arbeit:
Seite: 8, Zeilen: 10-19
Quelle: Tasdemir et al 2008
Seite(n): 613, 621, Zeilen: 613: r. col: 9 ff.; 621: r.col: 17 ff.
Contrary to monomeric AvGFP, all known Anthozoa GFP-like proteins reported to date form oligomers and exist mostly as tetramers (Baird et al., 2000; Mizuno et al., 2001; Wiedenmann et al., 2002; Shagin et al., 2004). The oligomerisation does not impair their application as reporter genes, selection markers or biosensors, but limits their use as fusion tags to study, for instance, protein localization and dynamics in living cells (Baird et al., 2000; Lauf et al., 2001; Mizuno et al., 2001). A possible solution to this problem is the genetic engineering of the fluorescent protein of interest by mutagenesis, creating mutants that form functional monomeric variants. For example, Tasdemir et al. (2008) converted the tetrameric AsGFP into dimeric and monomeric variants guided by a rational strategy of sequence alignments. Contrary to monomeric AvGFP, all known Anthozoa GFP-like proteins reported to date form oligomers and exist mostly as tetramers (Baird et al., 2000; Vrzheshch et al., 2000; Mizuno et al., 2001; Wiedenmann et al., 2002; Shagin et al., 2004). The oligomerisation does not impair their application as reporter genes, selection markers or biosensors, but limits their use as fusion tags to study, for instance, protein localisation and dynamics in living cells (Baird et al., 2000; Lauf et al., 2001; Mizuno et al., 2001). A possible solution to this problem is the genetic engineering of the fluorescent protein (FP) of interest by mutagenesis, creating mutants that form functional monomeric variants.

[page 621]

In summary, the present investigations led to a successful conversion of tetrameric AsGFP499 into dimeric and monomeric variants guided by a rational strategy of sequence alignments.

 Anmerkungen The source is mentioned, but as an illustration ("For example [...]") of the previously said and not as a reference of a source for it. Sichter (Hindemith), SleepyHollow02