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Typus
BauernOpfer
Bearbeiter
Hindemith
Gesichtet
Yes.png
Untersuchte Arbeit:
Seite: 71, Zeilen: 5-14
Quelle: Rahimi et al 2008
Seite(n): 5, Zeilen: 15 ff.
It is known that in the case of proteins only the fluorophores located at the surface can be dynamically quenched whereas the internal fluorophores are quenched by static process due to higher hydrophobicity in the interior. In the case of AsGFP, the chromophore is well shielded inside a β-barrel and hence is not accessible for dynamic or collisional quenching. Rahimi et al. (2008) reported for DsRed, that Cu2+ forms a complex with specific amino acids on the protein. This complex formation can lead to formation of non-fluorescent species in three ways, i) by affecting hydrogen-bonding network of the chromophore, ii) by bringing Cu2+ close to chromophore such that it contacts the excited-state of the chromophore, iii) by causing conformation/structural changes in the protein. It is known that in the case of proteins only the fluorophores located at the surface can be dynamically quenched whereas the internal fluorophores are quenched by static process due to higher hydrophobicity in the interior. In the case of DsRed, the chromophore is well shielded inside a β-barrel and hence is not accessible for dynamic or collisional quenching. [...] We hypothesize that, in DsRed, Cu2+ forms a complex with specific amino acids on the protein. This complex formation can lead to formation of non-fluorescent species in three ways, i) by affecting hydrogen-bonding network of the chromophore, ii) by bringing Cu2+ close to chromophore such that it contacts the excited-state of the chromophore, iii) by causing conformation/structural changes in the protein.
Anmerkungen

The source is mentioned in the middle. The reader might assume that the text following the reference is close to the source, but would expect that the text before the reference is the view of the author.

It is remarkable that Rahmini et al. (2008) "hypothesize that, in DsRed, Cu2+ forms a complex with specific amino acids on the protein", whereas T. M. writes: "Rahimi et al. (2008) reported for DsRed, that Cu2+ forms a complex with specific amino acids on the protein." From a scientific point of view, this is a significant misrepresentation.

Sichter
(Hindemith), SleepyHollow02

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