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| Untersuchte Arbeit: Seite: 15, Zeilen: 19-23, 26-27 |
Quelle: El Harrak 2009 Seite(n): 13, Zeilen: 8ff |
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| Experimental procedure
After minimum 30 min incubation, SD was induced by KCl application in temporal cortex. After another 15 min, several compounds were applied to the entorhinal cortex. Compounds applied were: DL-2-Amino-5-phosphonovaleric acid (APV; 50 μmol/L), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 μmol/L), N-Methyl-D-Aspartat (NMDA; 10 μmol/L) bicuculline (10 μmol/L), 4-aminopyridine (4-AP; 20 μmol/L), and WIN 55212-2 (5 μmol/L). NMDA, APV, CNQX, bicuculline also applied in accompanied with induction of LTP in the neocortex. The abovementioned drugs were administered focally through microelectrodes on to slices (electrode tip diameter 2–3 [μM, 0.5–2 bar; 500–800 ms, 3–5 nl).] |
Experimental procedure
After minimum 30 min incubation, CSD was induced by KCl application. After another 15 min, several compounds were applied to small areas of cortex between the two middle electrodes of the four-channel array. Compounds applied were: DL-2-Amino-5- phosphonovaleric acid (APV; 50 μmol/L), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 10 μmol/L), bicuculline (10 μmol/L), SKF 81297 (25 μmol/L), and quinpirole (20 μmol/L). The abovementioned drugs were administered focally through microelectrodes on to slices (electrode tip diameter 2–3 μM, 0.5–2 bar; 500–800 ms, 3–5 nl). |
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