von Dr. Vijay Pal Singh Rawat
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| [1.] Vpr/Fragment 035 01 - Diskussion Zuletzt bearbeitet: 2014-03-17 12:49:43 Graf Isolan | Fontanari Krause 2006, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Vpr |
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| Untersuchte Arbeit: Seite: 35, Zeilen: 1-14 |
Quelle: Fontanari Krause 2006 Seite(n): 92, 93, Zeilen: 92: 18-29 - 93: 1-2 |
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| 3.8 Cyto-Morphology.
Cyto-morphological analysis of bone marrow was done by transferring 5x104 to 1x105 bone marrow cells on glass slide by centrifugation at 500 rpm for 10 minute using a Shandon Cytospin2 centrifuge. Slides were stained with May- Grunwald’s eosine-methylene blue and Giemsa solution using the standard protocol supplied by Merck. 3.9 Immunophenotyping: Cell differentiation was determined and lineage distribution was checked by immunophenotyping. 5x104 to 1x104 cells were rinsed with PBS and stained for 30 minutes on ice with an appropriate concentration of phycoerythrin (PE) conjugated antibody to Gr-1, Sca1, Ter-119, CD4, and allophycocyanin-conjugated antibody for lineage markers Mac-1, cKit, B220 or CD8. The cells were then washed with PBS and stained with propidium iodide, and viable cells were analyzed with the FACS Calibur system. |
[page 92]
5.10.5. Cyto-Morphology Cyto-morphological analysis of bone marrow cells was done by transferring 5x104 to 1x105 bone marrow cells on glass slide by centrifugation at 500 rpm for 10 minute using a Shandon Cytospin2 centrifuge. Slides were stained with May-Grunwald’s eosine-methylene blue and Giemsa solution using the standard protocol supplied by Merck. 5.10.6. Immunophenotyping Cell differentiation was determined and lineage distribution was analyzed by immunophenotyping. 5x104 to 1x104 cells were rinsed with PBS and stained for 30 minutes on ice with an appropriate concentration of phycoerythrin (PE) conjugated antibody to Gr- 1, Sca-1, Ter-119, CD4, and allophycocyanin-conjugated antibody for lineage markers Mac-1, cKit, B220 or CD8 (all Pharmingen Heidelberg, Germany). The cells were then [page 93] washed with PBS and stained with propidium iodide, and viable cells were analyzed with the FACS Calibur system. |
The source is not given. |
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