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Typus
Verschleierung
Bearbeiter
SleepyHollow02
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Untersuchte Arbeit:
Seite: 37, Zeilen: 1-20
Quelle: Fontanari Krause 2006
Seite(n): 93, 94, Zeilen: 93: 20-27 - 94: 1-9
3.13 Analysis of sacrificed/dead experimental mice.

Disease in the animals was determined by analyzing PB, spleen and BM of diseased sacrificed mice. PB was aspirated from heart with 1 ml insulin syringes; Heparin was used for anticoagulation. PB was used for blood smears on glass slides and RBC count. WBC count and immunophenotyping was performed after lysing the RBC with ammonium chloride. The size of the spleen in diseased mice was measured and the spleen was homogenized to single cell suspension in DMEM medium, for further analysis by FACS, cytospin or ex vivo assays. BM cells were obtained by flushing both femurs and tibias and subjected to WBC count, cytospin and immunophenotyping.

3.14 Preparation for histopathology.

The four limbs, tail and head of mice were pinned to a cork plate with needles after sacrificing the animals for histological analyses. Then the skin from the chin to the anus and also the abdominal muscles were opened in the median and also pinned (Fig.16). The mice were then bled by cutting one of the renal arteries, and the blood was sucked out with a tissue paper. Then the diaphragm was carefully cut away to allow the fixing liquid to get into the thorax. After this the whole mouse was placed into formalin over night, packed and sent to the histopathology, where the organs were cut and stained for analyses (Fig.16).

[page 93]

5.10.8. Analysis of sacrificed/dead experimental mice

The PB, spleen and BM of sacrificed mice were analyzed carefully. PB was aspirated from heart with 1 ml insulin syringes; Heparin was used for anticoagulation. PB was used for blood smears on glass slides and RBC count. WBC count and immunophenotyping was performed after lysing the RBC with ammonium chloride. The spleen size was measured in diseased mice, and then homogenized for single cell suspension in DMEM medium, for further analysis by FACS, cytospin or ex vivo assays. BM cells were obtained by flushing both femurs and tibias and subjected to WBC count, cytospin and immunophenotyping.

[page 94]

5.10.9. Preparation for histopathology

The four limbs, tail and head of mice were pinned to a cork plate with needles after sacrificing the animals for histological analyses. Then the skin from the chin to the anus and also the abdominal muscles were opened in the median and also pinned down (Fig.2.3). The mice were then bled by cut one of the renal arteries, and the blood was sucked out with a tissue paper. Then the diaphragm was carefully cut away to allow the fixing liquid to penetrate into the thorax. After this the whole mouse was placed into formalin (37%) over night, packed and sent to the department of pathology (PD. Dr. Leticia Quintanilla-Fend), where the organs were cut and stained for analyses.

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