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Genetic Diversity of Helicobacter pylori Isolates in Sudan

von Dr. Wael Faroug Elamin

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[1.] Wfe/Fragment 006 01 - Diskussion
Zuletzt bearbeitet: 2016-01-09 22:04:32 Hindemith
Fragment, Gesichtet, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Wanken 2003, Wfe

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Hindemith
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Untersuchte Arbeit:
Seite: 6, Zeilen: 1-24
Quelle: Wanken 2003
Seite(n): 11, 12, 13, Zeilen: 11: 13ff; 12: 9ff; 13: 1ff
Therefore, the presence or absence of the cag PAI is an important characteristic among H. pylori strains.

Additional strain-specific genes have been identified by comparing the complete genome sequences of H. pylori 26695 with H. pylori J99 (Alm and Trust , 1999). The overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genome) were found to be quite similar. Both strains contained the complete cag PAI flanked by the same chromosomal genes and a previously described 31-bp repeat (Akopyants et al., 1998; Censini et al., 1996). The DNA-sequence differences between orthologues from the two strains are found mainly in the third position of coding triplets, consistent with the variation seen between H. pylori strains identified by methods dependent on the nucleotide sequence or on the sequencing of specific loci in different strains (Akopyanz [sic] et al., 1992). This nucleotide variation however does not translate into a highly divergent proteome. A total of 275 (18.4%) J99 and 290 (18.7%) 26695 gene products have orthologues of unknown function in other species, and 346 (23.1%) J99 and 367 (23.6%) 26695 genes are H. pylori specific, showing no sequence similarity with genes available in public databases. Of these H. pylori specific genes, 56 and 69 are specific to strains J99 and 26995, respectively (Alm et al., 1999).

The fact that strain-specific DNA-restriction/modification genes have a lower (G+C) content than the remainder of the genome and are associated with regions that are organized differently in the J99 and 26695 genomes indicates that these genes may have been acquired horizontally from other bacterial species or transferred more recently from other H. pylori strains by natural transformation (Alm et al., 1999).


Alm, R. A., and T. J. Trust, Analysis of the genetic diversity of Helicobacter pylori: the tale of two genomes, J Mol Med, 77, 834-846, 1999.

Alm, R. A., L. S. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust, Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori, Nature, 397, 176-180, 1999.

Akopyanz, N., N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg, DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based rapd fingerprinting, Nucleic Acids Res, 20, 5137-5142, 1992.

Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg, Analyses of the cag pathogenicity island of Helicobacter pylori, Mol Microbiol, 28, 37-53, 1998.

Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovsky, R. Rappuoli, and A. Covaeci, cag. a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors, Proc Natl Acad Sci USA, 93, 14,648-14,653, 1996.

Therefore, the presence or absence of the cag PAI is an important characteristic among H. pylori strains.

[page 12]

Additional strain-specific genes have been identified by comparing the complete genome sequences of H. pylori 26695 with H. pylori J99 (7). The overall genomic organization, gene order and predicted proteomes (sets of proteins encoded by the genome) were found to be quite similar. Both strains contained the complete cag PAI flanked by the same chromosomal genes and a previously described 31-bp repeat (2). The DNA-sequence differences between orthologues from the two strains are found mainly in the third position of coding triplets, consistent with the variation seen between H. pylori strains identified by methods dependent on the nucleotide sequence or on the sequencing of specific loci in different strains (1). However, this nucleotide variation does not translate into a highly divergent proteome. A total of 275 (18.4%) J99 and 290 (18.7%) 26695 gene products have orthologues of unknown function in other species, and 346 (23.1%) J99 and 367 (23.6%) 26695 genes are H. pylori specific, showing no sequence similarity with genes available in public databases. Of these H. pylori specific genes, 56 and 69 are specific to stains J99 and 26995, respectively (7).

[page 13]

The fact that strain-specific DNA-restriction/modification genes have a lower (G+C) content than the remainder of the genome and are associated with regions that are organized differently in the J99 and 26695 genomes indicates that these genes may have been acquired horizontally from other bacterial species or transferred more recently from other H. pylori strains by natural transformation (7).


1. Akopyants, N., N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg. 1992. DNA diversity among clinical isolates of Helicobacter pylori deteted [sic] by PCR-based RAPD fingerprinting. Nucleic Acids Res. 20:5137-5142.

2. Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol. Microbiol. 28:37-53.

7. Alm, R., L.-S. L. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180.

Anmerkungen

The source is not mentioned

Sichter
(Hindemith) Schumann


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